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Axio imager m2 compound microscope

Manufactured by Zeiss
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The Zeiss Axio Imager M2 is a compound microscope designed for high-resolution imaging and analysis. It features a modular design, allowing for customization to meet specific research needs. The microscope is equipped with advanced optics and illumination systems to provide clear, detailed images across a range of sample types.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (3 mentions) Lsm 700 confocal microscope, by Zeiss (2 mentions) Alexa fluor 555 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions) Cm1850 cryostat, by Leica (1 mentions) Rabbit anti acetylated tubulin, by Cell Signaling Technology (1 mentions) Zen 2009 le software, by Zeiss (1 mentions) Seaplaque, by Lonza (1 mentions) Rm2145, by Leica (1 mentions)

Close spelling variants of this product

Axio Imager M2 (1035 citations) Axio Imager (671 citations) Axio Imager M2 microscope (593 citations) Imager M2 (184 citations) Axio microscope (122 citations) Imager M2 microscope (106 citations) Compound microscope (36 citations) M2 microscope (29 citations) Axio M2 microscope (6 citations) Imager M2 compound microscope (2 citations)

6 protocols using axio imager m2 compound microscope

1

Scoring Neuronal Defects in Live Animals

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A Zeiss Axio Imager M2 compound microscope was used for visual scoring of defects in neuronal morphology of live animals. Animals were anesthetized using 5 mM levamisole in M9 buffer. Markers used are: Pmec-4-GFP(zdIs5), Pmec-7-GFP(muIs32), and Punc-25-GFP(juIs76). TRN morphology was scored using zdIs5 unless otherwise noted. In scoring ALM ectopic neurite outgrowth, any projections from the ALM soma other than the anterior axon were measured and scored as “ectopic posterior neurite” if longer than 10 μm. ‘Multiple ectopic neurites’ was defined as >1 ectopic neurite extending from the ALM soma. PLM overshooting was defined as the axon of PLM extending anterior to the ALM soma13 (link). ALM soma shape was scored as normal (0), mildly defective (1) or severely defective (2) as detailed in Figure 2 legend.
Park S., Noblett N., Pitts L., Colavita A., Wehman A.M., Jin Y, & Chisholm A.D. (2024). Dopey-dependent regulation of extracellular vesicles maintains neuronal morphology. bioRxiv.
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2

Microscopy Imaging and Statistical Analysis

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H&E and AB-PAS samples were imaged using a Zeiss AXIO Imager M2 compound microscope. An LSM900 Confocal Zeiss microscope was used to image immunofluorescent-stained samples, which were then analyzed using Fiji software [29 (link)]. The data were put through a Shapiro–Wilk normality test and a Levene test for equality of variances. Based on the normality and variance homogeneity of the datasets, Student’s t-test, Welch’s t-test, or Mann–Whitney U-test were applied to determine statistical significance. Student’s t-test was used for ordinal datasets.
Li Y., Lee A.Q., Lu Z., Sun Y., Lu J.W., Ren Z., Zhang N., Liu D, & Gong Z. (2022). Systematic Characterization of the Disruption of Intestine during Liver Tumor Progression in the xmrk Oncogene Transgenic Zebrafish Model. Cells, 11(11), 1810.
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3

Fluorescent Immunohistochemistry and in situ Hybridization Protocol

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Cryo-sectioning of 14µm slices followed by fluorescent immunohistochemistry was performed on a Leica Cryostat CM1850. As primary antibodies rabbit anti-Acetylated Tubulin (Cell Signaling Technologies, 5335T) and mouse anti-Elavl3/Huc (Thermo Fisher Scientific, A-21271) were used in dilutions of 1:500 in blocking medium. As secondary antibodies goat-anti-mouse AlexaFluor-488 (ThermoFisher Scientific, A-11001) and goat-anti-rabbit AlexaFluor-555 (ThermoFisher Scientific, A-21428) were used in dilutions of 1:1000. After mounting with Dapi inoculated Moviol, samples were imaged on a Zeiss LSM710 confocal microscope.
Whole-mount in situ hybridizations were performed as previously described.28 (link) Stained specimens were cleared in 80% glycerol/PBS for several hours followed by mounting in 100% glycerol for image capturing on a Zeiss AxioImagerM.2 compound microscope using a 10x objective. Riboprobes of isl1 and atho7 cDNA were generated and used as described.29 (link),30 (link) Calculation of statistical significance among compared groups of certain experimental conditions was done via a χ2-test utilizing the programming language R. To allow for χ2-testing in this context algorithmic operations were performed with the three sub-groups (I) strong, (II) medium, (III) weak+none (sub-groups weak and none of the chart in Fig 5 L–L’’’ were merged into a single phenotypic class).
Jurkute N., Leu C., Pogoda H.M., Arno G., Robson A.G., Nürnberg G., Altmüller J., Thiele H., Motameny S., Toliat M.R., Powell K., Höhne W., Michaelides M., Webster A.R., Moore A.T., Hammerschmidt M., Nürnberg P., Yu-Wai-Man P, & Votruba M. (2019). SSBP1 mutations in dominant optic atrophy with variable retinal degeneration. Annals of neurology, 86(3), 368-383.
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4

Zebrafish Angiogenesis Imaging Protocol

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Tg(fli1:EGFP)y1 zebrafish were used in this study [34 (link)]. To block pigmentation, embryos were raised from 22 hpf in the presence of 0.003% N-Phenylthiourea (PTU; Sigma-Aldrich #P7629). 29 hpf embryos were placed in each well of a 24-well plate in 1 mL egg water containing 0.1% DMSO (control) or 500 µM TNP-470. Embryos were kept at 28.5 °C until imaging took place. For imaging, live embryos were anaesthetized using Tricaine mesylate and were mounted in 0.5% low-melting-point agarose (SeaPlaque, Lonza). Images were acquired using a Zeiss LSM700 confocal microscope and an Axio Imager M2 compound microscope equipped with a 40 × 1.0 NA water objective. Images were exported as TIFF files using ZEN 2009 LE software (Zeiss), and figures were assembled using the Adobe Photoshop CS4 software, n = 17.
Esa R., Steinberg E., Dror D., Schwob O., Khajavi M., Maoz M., Kinarty Y., Inbal A., Zick A, & Benny O. (2020). The Role of Methionine Aminopeptidase 2 in Lymphangiogenesis. International Journal of Molecular Sciences, 21(14), 5148.
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5

Histopathological Analysis of Excision Wound

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On the 15th post-wounding day, the animal skin tissue specimens of corresponding groups in the excision wound model were collected to perform histopathological studies. Skin specimens were processed and fixed in Bouin’s solution, followed by paraffin blocking. Skin sections of 5 µm thickness were taken in a microtome (Leica, RM 2145), followed by staining with hematoxylin and eosin (H&E), Masson’s trichome, and Toluidine blue stains as per standard procedures (Suntar et al., 2010 (link)). The microphotographs of sections were captured through an inbuilt analog camera (ProgRess® C5-JENOPTIK) attached to Carl Zeiss Axio Imager M2 compound microscope and the images were analyzed at (×100 and ×400 magnifications. Quantification of different cells (neutrophils, inflammatory cells, blood vessels, myofibroblasts, mast cells, and macrophages) per unit area of images at ×100 magnification were performed using NIH ImageJ software (Hashemnia et al., 2019 (link)).
Bhat P., Patil V.S., Anand A., Bijjaragi S., Hegde G.R., Hegde H.V, & Roy S. (2023). Ethyl gallate isolated from phenol-enriched fraction of Caesalpinia mimosoides Lam. Promotes cutaneous wound healing: a scientific validation through bioassay-guided fractionation. Frontiers in Pharmacology, 14, 1214220.
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6

Analyzing Intestinal GFP and LGG-1 Puncta

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Young adult animals were transferred to NGM plates containing 50 or 300 mM NaCl and 0 or 400 µM FUdR for 48 hours. After treatment, animals were mounted on an agarose pad and paralyzed for 5 minutes with 0.03% sodium azide. Images of GFP fluorescence were obtained on a Zeiss AxioImager M2 compound microscope, using 3ms exposures and identical settings for all images in a set. Fluorescent intensity was quantified in a uniform area of the anterior and posterior intestine for each image, using Axiovision 4.8 software. Differences in mean fluorescence intensity were compared with Student’s t-test. LGG-1::GFP puncta were counted in seam cells of 2-day old adults. Differences in numbers of puncta per cell were compared with the Wilcoxon rank-sum test.
Anderson E.N., Corkins M.E., Li J.C., Singh K., Parsons S., Tucey T.M., Sorkaç A., Huang H., Dimitriadi M., Sinclair D.A, & Hart A.C. (2016). C. elegans lifespan extension by osmotic stress requires FUdR, base excision repair, FOXO, and sirtuins. Mechanisms of ageing and development, 154, 30-42.
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