Oil red o kit

Phosphate buffer (PBS, 0.1 mM, pH 6.8), dimethyl sulfoxide (DMSO), formaldehyde and sodium carbonate (Na₂CO₃) were purchased from Chengdu Kelong Chemical Reagent Factory (Chengdu, China); α-glucosidase and 4-nitrophenyl-beta-D-glucopyranoside (PNPG) were purchased from Rhawn Chemical Reagent Company (Shanghai, China); fetal bovine serum (FBS) was purchased from HyClone Inc (Utah, USA); the Oil Red O kit and glucose assay kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China); the Cell Counting Kit-8 Cell (CCK-8) proliferation-toxicity test kit was purchased from Boster Biological Technology Company (Wuhan, China); human insulin was purchased from Sigma-Aldrich (St. Louis, MO, USA); high-glucose DMEM was purchased from GIBCO (New York, USA), radioimmunoprecipitation assay (RIPA) lysate buffer, bicinchoninic acid (BCA) protein quantitative kit, and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) preparation kit were purchased from Multi Sciences (Hangzhou, China); primary antibodies for AMPK, phosphorylation- (p-) AMPK, PI3K, p-PI3K, Akt, and p-Akt were obtained from the ImmunoWay Biotechnology Co. (Suzhou, China), horseradish peroxidase- (HPR-) conjugated secondary antibody were purchased from the Beyotime Institute of Biotechnology (Haimen, China).