Anti cd57 fitc

EDTA whole blood samples from 5 healthy subjects, 10 patients with mild and 10 patients with severe disease were stained with anti-HLA-DR PE and ECD, anti-CD3 ECD, anti-CD4 PE, anti-CD8 FITC, anti-CD19 PC7, anti-CD14 FITC, anti-CD16 PC5, anti-CD15 PE, anti-CD57 FITC, anti-CD56 PE, anti-CD11c PCP, anti-CD123 PE, anti-CD83 PE, anti-CD38 PE, anti-CD23 ECD and isotype controls (all from Beckman Coulter) for 20 minutes in the dark at +4°C. Samples were analyzed on the flow cytometer Cytomics FC500 (Beckman Coulter). Data were processed by FlowJo V.10.

Whole blood samples (100 μl) were stained with anti-CD3 ECD, anti-HLA-DR PE (or ECD) (Invitrogen), anti-CD4 PE (or ECD), anti-CD8 FITC, anti-CD19 FITC (or PE), anti-CD14 FITC (or PC5), anti-CD15 PE, anti-CD57 FITC (Beckman Coulter) and isotype controls (Beckman Coulter) for 25 min. at +4°C. Intracellular staining with anti-Foxp3 (eBioscience) was performed after surface staining with anti-CD4 PE, followed by fixation and permeabilisation with Foxp3-Transcription Factor Staining Buffer Set (eBioscience) according to the manufacturer’s recommendations.
Samples were acquired on a Cytomics FC500 (Beckman Coulter) and analyzed with Flowing Software (version 2.5.1; Turku Centre for Biotechnology, University of Turku, Finland [http://www.flowingsoftware.com/]).