Lab grade stearic acid

Lab grade stearic acid was purchased from Merck (© Merck KGaA, Darmstadt, Germany) and excipient grade stearic and Tween^® 60 were obtained from Acofarma (^® Acofarma–Acofarma Distribución, S.A. BCN, Spain). Arachidic acid, lab grade Tween^® 60, l-lysine monohydrochloride, lithium carbonate, dansyl chloride, ethylenediaminetetraacetic (EDTA) acid disodium salt dihydrate, dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Triton^tm X-100, methylamine hydrochloride, triethylamine, sodium acetate, pepsin and pancreatin (from porcine pancreas) were purchased from Sigma-Aldrich (St. Luis, MO, USA), l-phenylalanine ethyl-ester hydrochloride from Fluka (Fluka Chemie GmbH, Buchs, Switzerland), acetic acid from VWR Chemicals (VWR International S.A.S., Fontenay-sous-Bois, France), acetonitrile and methanol from Honeywell (Honeywell Riedel-de Häen AG, Seelze, Germany). Dulbecco’s Modified Eagle’s Medium (DMEM), penicillin–streptomycin (10,000 U/mL) mix, fetal bovine serum (FBS) and amphotericin B (Fungizone) were purchased from Gibco^® (Invitrogen Corporation, UK). FaSSGF and FeSSIF were purchased from Biorelevant.com Ltd (London, UK). Aqueous solutions were prepared with double-deionized water (Arium Pro, Sartorius AG, Göttingen, Germany).

Lab grade stearic acid was purchased from Merck (© Merck KGaA, Darmstadt, Germany) and excipient grade stearic and Tween^® 60 were obtained from Acofarma (^® Acofarma–Acofarma Distribución, S.A. BCN, Spain). Arachidic acid, lab grade Tween^® 60, l-lysine monohydrochloride, lithium carbonate, dansyl chloride, ethylenediaminetetraacetic (EDTA) acid disodium salt dihydrate, dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Triton^tm X-100, methylamine hydrochloride, triethylamine, sodium acetate, pepsin and pancreatin (from porcine pancreas) were purchased from Sigma-Aldrich (St. Luis, MO, USA), l-phenylalanine ethyl-ester hydrochloride from Fluka (Fluka Chemie GmbH, Buchs, Switzerland), acetic acid from VWR Chemicals (VWR International S.A.S., Fontenay-sous-Bois, France), acetonitrile and methanol from Honeywell (Honeywell Riedel-de Häen AG, Seelze, Germany). Dulbecco’s Modified Eagle’s Medium (DMEM), penicillin–streptomycin (10,000 U/mL) mix, fetal bovine serum (FBS) and amphotericin B (Fungizone) were purchased from Gibco^® (Invitrogen Corporation, UK). FaSSGF and FeSSIF were purchased from Biorelevant.com Ltd (London, UK). Aqueous solutions were prepared with double-deionized water (Arium Pro, Sartorius AG, Göttingen, Germany).