Diatome ultra 45 diamond knife

Manufactured by Electron Microscopy Sciences

Sourced in Germany

The Diatome ultra 45° diamond knife is a precision cutting tool designed for ultramicrotomy. It features a 45-degree cutting angle and is made with high-quality diamond material.

Automatically generated - may contain errors

Lab products found in correlation

Em uc7 ultramicrotome, by Leica (2 mentions) Morgagni 268, by Thermo Fisher Scientific (2 mentions) Dsc hx1 camera, by Sony (1 mentions) Rm2255 microtome, by Leica (1 mentions) H600 tem microscope, by Hitachi (1 mentions) G200h cu, by Electron Microscopy Sciences (1 mentions) Embed 812 resin, by Electron Microscopy Sciences (1 mentions)

Close spelling variants of this product

DiATOME 45° diamond knife Ultra 45° diamond knife Diatome diamond knife 45° diamond knife Ultra-diamond knife Diamond knife Ultra 45°, Diatome

3 protocols using diatome ultra 45 diamond knife

Ultrastructural Analysis of Thyroid Tissue

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For transmission electron microscopy (TEM), one thyroid lobe was removed from two randomly chosen animals per group, sliced in 4% glutaraldehyde solution in 100 mM phosphate buffer, pH 7.4, for 24 h at 4 °C, and further processed as previously described [30 (link)]. In brief, post fixation was carried out with 1% OsO4 for 1 h at 4 °C, and counterstaining with uranyl acetate. Samples were dehydrated through a graded series of ethanol and embedded in Araldite resin. A Leica EM UC7 ultramicrotome (Leica, Germany) with a Diatome ultra 45° diamond knife (Diatome, Switzerland) was used for cutting ultrathin sections of thyroid tissue at a thickness of 70 nm. Grids with ultrathin sections were stained with uranyl acetate and lead citrate and examined under a Morgagni 268 (FEI Company, The Netherlands) transmission electron microscope.
The number lysosomes in thyrocytes was analyzed on TEM micrographs manually, while their diameter was measured by using Windows based ImageJ (Image J, Version 1.49j). Measurements were conducted on 10 thyrocytes per group.

Šošić-Jurjević B., Trifunović S., Živanović J., Ajdžanović V., Miler M., Ristić N, & Filipović B. (2022). Vitamin D3 Treatment Alters Thyroid Functional Morphology in Orchidectomized Rat Model of Osteoporosis. International Journal of Molecular Sciences, 23(2), 791.

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Craniofacial Ultrastructure Analysis

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4dpf embryos were fixed in cacodylate fix (0.1M cacodylate, 2% PFA, 2% glutaraldehyde [pH 7.4]) at 4°C overnight, postfixed in 1% osmium buffer solution, dehydrated through a series of methanol and acetonitrile washes, and infiltrated with Embed 812 resin (14120; Electron Microscopy Sciences, Hatfield, PA). Semithin transverse sections (1 µm) were cut with glass knives on a Leica RM2255 microtome, heat-fixed to glass slides, stained with 1% toluidine blue in 1% borax buffer, and imaged on a Nikon Eclipse E800 light microscope with an attached Sony DSC HX1 camera to capture craniofacial images. Plastic blocks were then submitted to the Electron Microscopy Facility at the Medical College of Wisconsin for electron microscopy. Ultrathin sections (70–80 nm) were collected using a DiATOME Ultra 45° diamond knife (MT7376; DiATOME), collected on copper hexagonal mesh coated grids (G200H-Cu; Electron Microscopy Sciences), and stained with uranyl acetate and lead citrate for contrast. Images were captured using a Hitachi H600 TEM microscope (Hitachi, Tokyo, Japan).

Clark E.M, & Link B.A. (2021). Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis. Molecular Biology of the Cell, 32(5), 391-401.

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Ultrastructural Analysis of Thyroid Tissue

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For the ultrastructural analysis, the thyroid lobe was removed from two animals per group and processed as previously described (Živanović et al., 2019) . The thyroid lobe was sliced in 4% glutaraldehyde solution in 100 mM phosphate buffer, pH 7.4, for 24 h. Postfixation was carried with 1% OsO4 for 1 h at 4 °C, and counterstaining with uranyl acetate. Samples were dehydrated through a graded series of ethanol and embedded in Araldite resin. A Leica EM UC7 ultramicrotome (Leica, Germany) with a Diatome ultra 45°diamond knife (Diatome, Switzerland) was used for cutting ultrathin sections of thyroid tissue at a thickness of 70 nm. Grids with ultrathin sections were stained with uranyl acetate and lead citrate and examined under a Morgagni 268 (FEI Company, Eindhoven, The Netherlands) transmission electron microscope.

Miler M., Ajdžanović V., Živanović J., Marković Filipović J., Šošić-Jurjević B, & Milošević V. (2021). Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study. Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 27(2).

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