Dri chem 7000 chemistry analyzer

Single mice were kept for 24-hours in metabolic cages (3600M021, Techniplast) and urine and stool production, as well as water and food intake were measured. Treatments were performed with dDAVP (0.4μg/kg intraperitoneally) and effects on these parameters were assessed. Mouse serum chemistries were determined with a Dri-Chem7000 chemistry analyzer (Heska). Urine chemistries were determined with a Roche ModP analyzer and urine osmolality with an Advanced Instruments Micro Osmometer Model 3300. CBC was obtained with the HemaTrue analyzer (Heska). Blood gas analysis was performed with an IRMA TRUpoint capillary blood analysis system (Lifehealth). Mouse serum renin was measured with a mouse renin 1 ELISA kit (EMREN1, Thermo Fisher), according to the manufacturer’s instructions. Aldosterone assays were performed with a commercially available radioimmunoassay kit (ACTIVE® Aldosterone RIA, Beckman Coulter, IMMUNOTECH, Prague, Czech Republic). Anti-aldosterone antibody-coated tubes, ¹²⁵I-labeled aldosterone radioligand, 7 calibrators ranging from 0 to 4448 pmol/L, and 2 controls were provided by the manufacturer. The assays were performed according to the kit protocol with the modification of an overnight incubation step at room temperature.

Serum blood urea nitrogen (BUN) and creatinine were examed for kidney injury and alanine aminotransferase (ALT) and aspartate aminotransferase (AST) for liver injury. Blood was collected 24 hours after sham or CLP surgery. Serum was prepared and stored at −80 °C until analysis. Serum chemistry was measured by DRI-CHEM 7000 Chemistry Analyzer (HESKA, Des Moines, IA) according to the manufacturer’s instruction.