Biotin‐conjugated anti‐CD41 monoclonal antibody was from Abcam. Recombinant human Annexin V was from Enzo Life Sciences. SP600125 was from Calbiochem. The antibodies rabbit anti‐MKK4, p‐MKK4, c‐JNK and mouse anti‐p‐JNK were from Cell Signaling Technology. Phycoerythrin (PE)‐conjugated anti‐CD36 (clone CB38), PE‐conjugated CD36 isotype control (clone G155‐228), PE‐cy™5‐conjugated mouse anti‐CD41a antibody (clone HIP8) and fluorescein isothiocyanate (FITC)‐conjugated PAC‐1 were from BD Biosciences/Pharmingen. The 8‐iso‐prostaglandin‐F2α (8‐iso‐PG‐F2α) enzyme immunoassay (EIA) kit was from Cayman Chemical. The p‐selectin ELISA kit was from R&D Systems. The MV‐capture antibody AD‐1 was originally raised against human membrane‐bound liver alkaline phosphatase isolated from patients with liver cancer.10 , 11 The hybridoma cells were cultured in RPMI 1640 medium containing 10% FBS to produce antibody.
Fluorescein isothiocyanate fitc conjugated pac 1
Manufactured by BD
Sourced in United States, United Kingdom
Fluorescein isothiocyanate (FITC)-conjugated PAC-1 is a fluorescent-labeled compound used in laboratory research applications. It is a derivative of the protein PAC-1, which is conjugated to the fluorescent dye FITC. This product is intended for use in various experimental techniques that require fluorescent labeling, such as flow cytometry, immunohistochemistry, and fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
P mkk4, by Cell Signaling Technology (1 mentions)
P selectin elisa kit, by R&D Systems (1 mentions)
Rabbit anti mkk4, by Cell Signaling Technology (1 mentions)
Mouse anti p jnk, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Antibodies against caspase 3, by Santa Cruz Biotechnology (1 mentions)
Bcl xl, by Santa Cruz Biotechnology (1 mentions)
Par1 ap sfllrn amide, by Bachem (1 mentions)
Par4 ap aypgkf amide, by Bachem (1 mentions)
Cal 520 am, by AAT Bioquest (1 mentions)
U46619, by Apexbio (1 mentions)
3 protocols using fluorescein isothiocyanate fitc conjugated pac 1
1
Platelet activation biomarkers analysis
2
Apoptosis Induction Assay Protocol
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Fluorescein isothiocyanate (FITC)-conjugated PAC-1 was purchased from BD Bioscience (CA, USA). The 5,5',6,6'-tetrachloro-1,1',3,3'tetraethylbenzimidazolcarbocyanine iodide (JC-1) and E64 were purchased from Roche (Indianapolis, IN, USA). FITC-conjugated annexin V was purchased from Jiamay Biotech (Beijing, China). FITC-conjugated anti-mouse CD62P antibody and anti-human CD62P antibody were from Biolegend (San Diego, CA, USA). Antibodies against Caspase-3, Bax, Bak, and Bcl-XL were purchased from Santa Cruz (Santa Cruz, CA, USA). Anti-β-actin was purchased from Sigma-Aldrich (LA, USA). The purified TSST-1 protein was purchased from Toxin Technology Inc (Sarasota, USA) with an over 98% purity.
3
Platelet Activation Assay Protocol
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Chemicals were obtained from Sigma Aldrich (UK) unless otherwise stated. Q94 was obtained from Tocris (Bristol, UK) and ChemDiv (San Diego, USA). Vorapaxar and ML161 were from Generon (Slough, UK). PAR1-AP (SFLLRN-amide) and PAR4-AP (AYPGKF-amide) were from Bachem (Weil am Rhein, Germany). U46619 was from ApexBio (Stratech, Cambridge, UK). Cal520-AM was from AAT Bioquest (Stratech, Cambridge, UK). Fluorescein isothiocyanate (FITC)-conjugated PAC-1 and phycoerythrin (PE)conjugated anti-CD62P antibodies were from BD Biosciences (Wokingham, UK).
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