Autophagosomes were detected by a Cyto-ID staining kit (ENZO Life Science, New York, USA) according to the manufacturer’s protocol [23 (link)]. THP-1 cells grown on 12-well plates, cells were treated with 100uM of CPPecp at 37°C for a time course study, and the cells were also treated with 10uM of tamoxifen for six hours was used as autophagy induction positive control. After treatment, the medium was removed with testing reagents and positive control and the cells were washed twice with 1X assay buffer. One hundred μL of Microscopy Dual Detection Reagent was dispensed to cover each sample of monolayer cells. Samples were kept in the dark and incubated for 15–30 minutes at 37°C. Cells were then washed with 100 μL of 1X assay buffer. Stained cells were analyzed by flow cytometry (BD FACSCalibur).
Cyto id staining kit
Manufactured by Enzo Life Sciences
Sourced in United States
The Cyto-ID staining kit is a fluorescent dye-based tool used to detect and quantify cellular processes in live cells. It provides a simple and reliable method for monitoring autophagic activity.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cyto id staining kit
1
Autophagosome Detection in THP-1 Cells
2
Autophagy Detection in Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cyto-ID staining kit (Enzo Life Sciences, Farmingdale, NY) was used to detect autophagy in HEK293, Nthy-Ori-3 and TPC1 cells according to the manufacturer’s protocol. Briefly, cells were plated on cover slips at 70% confluency and allowed to attach overnight. Cell medium was aspirated followed by carefully washing twice using 1x Assay Buffer with 5% FBS. Cells were incubated with Cyto-ID Green Detection Reagent for 30 min at 37ºC in the dark. Cells were carefully washed twice with 1X Assay Buffer to remove excess dye and observed immediately under a confocal microscope.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!