Hiseq 2500 150 pe

The whole-genome amplified products or genomic DNA were fragmented using an EpiSonic Multi-Functional Bioprocessor 1100 (Epigentek) to generate DNA fragments with 250-bp on average. The fragmented products underwent Illumina library preparation using Celemics NGS Library Preparation Kit (LI1096, Celemics, Seoul, Korea) for the whole-genome sequencing library preparation, and SureSelectXT (Agilent, CA, US) for whole-exome sequencing. DNA purification was performed by TOPQXSEP MagBead (XB6050, Celemics, Seoul, Korea), and DNA libraries were amplified using the KAPA Library Amplification Kit (KAPA Biosystems, KK2602). Finally, the products were quantified by TapeStation 2200 (Agilent, CA, US). We used HiSeq 2500 150 PE (Illumina) to generate 1 Gb/sample for whole-genome sequencing and 5 Gb/sample for whole-exome sequencing, respectively.

The whole-genome-amplified products or genomic DNA extracts were fragmented using an EpiSonic Multi-Functional Bioprocessor 1100 (Epigentek) to generate a 150~250-bp fragment distribution. The fragmented products underwent Illumina library preparation for end repair, 3′dA-tailing, adaptor ligation, and PCR amplification according to the manufacturers’ instructions. We used the Celemics NGS Library Preparation Kit (LI1096, Celemics, Seoul, Korea) for the whole-genome sequencing library preparation, SureSelectXT (Agilent, CA, USA) for whole-exome sequencing, and the Celemics Customized Target Enrichment Kit (SICT96, Celemics, Seoul, Korea) for targeted sequencing. DNA purification was performed by ^TOPQXSEP MagBead (XB6050, Celemics, Seoul, Korea), and DNA libraries were amplified using the KAPA Library Amplification Kit (KAPA Biosystems, KK2602). Finally, the products were quantified by TapeStation 2200 (Agilent, CA, USA). We used a HiSeq 2500 50SE (Illumina) to generate 0.16 Gb/sample for whole-genome sequencing and a HiSeq 2500 150PE (Illumina) to generate 5 G/sample and 0.88G/sample for whole-exome and targeted sequencing, respectively.