AF-488 (1:500) labelled-Tf and AF-647 labelled WGA (1:200) were from Invitrogen (Thermo Fisher Scientific); siR-Tubulin (1:1,000) was from Spirochrome. PAO (PhenylArsineOxide), wortmannin, GSK-A1, and rapamycin were from Sigma Aldrich, and stock solutions were dissolved in dimethyl sulfoxide (DMSO; Sigma Aldrich). The following reagents for in vitro studies were from Avanti Polar Lipids: Egg phosphatidylcholine, brain phosphatidylserine, and 1,2-dioleoyl-sn-glycero-3-phospho-(1'-myo-inositol-3'-phosphate) 18:1 (EPC, bPS, and PI3P, respectively; in chloroform); brain phosphatidylinositol-4-phosphate (bPI4P; in chloroform/methanol/water [20:9:1]); brain phosphatidylinositol-4,5-bisphosphate, C24:1 Galactosyl(β) Ceramide, and 1,2-dioleoyl-sn-glycero-3-phospho-l -serine (bPI[4,5]P2, GalCer, and DOPS; in chloroform/methanol [90/10% v/v]). Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Texas Red DHPE) and BODIPY Texas Red ceramide (BODIPY TR ceramide) was from Thermo Fisher Scientific.
Texas red 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine triethylammonium salt texas red dhpe
Manufactured by Thermo Fisher Scientific
Sourced in United States
Texas Red DHPE is a fluorescent lipid probe used for labeling and visualization of cellular membranes. It consists of a Texas Red fluorophore covalently attached to a phosphoethanolamine lipid. The Texas Red dye has excitation and emission maxima of 595 nm and 615 nm, respectively, allowing for detection in the red fluorescence channel.
Automatically generated - may contain errors
Lab products found in correlation
1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Wortmannin, by Merck Group (1 mentions)
Gsk a1, by Merck Group (1 mentions)
Brain phosphatidylserine, by Avanti Polar Lipids (1 mentions)
Egg phosphatidylcholine, by Avanti Polar Lipids (1 mentions)
1 2 dioleoyl sn glycero 3 phospho 1 myo inositol 3 phosphate, by Avanti Polar Lipids (1 mentions)
Sir tubulin, by Spirochrome (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Poly l lysine (pll), by Merck Group (1 mentions)
Polyethyleneimine, by Merck Group (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phospho 1 rac glycerol sodium salt popg, by Avanti Polar Lipids (1 mentions)
Hydrochloric acid (hcl), by Fujifilm (1 mentions)
Methanol, by Fujifilm (1 mentions)
Sodium hydroxide (naoh), by Fujifilm (1 mentions)
Uranine, by Fujifilm (1 mentions)
Chloroform, by Fujifilm (1 mentions)
Fructose, by Fujifilm (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
6 protocols using texas red 1 2 dihexadecanoyl sn glycero 3 phosphoethanolamine triethylammonium salt texas red dhpe
1
Fluorescent Labeling Reagents for Cell Imaging
2
Preparation of Lipid Membrane and Microfluidic Device
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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine
(POPC) was purchased from NOF Corporation (Tokyo, Japan). Cholesterol
was provided by Avanti Polar Lipids. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (triethylammonium salt)
(Texas Red DHPE) was supplied by Thermo Fisher Scientific, Inc. (Waltham,
MA). Glucose, sucrose, tris(hydroxymethyl)aminomethane (Tris), hydrochloric
acid, methanol, chloroform, hexadecane, liquid paraffin, and pH standard
solutions (phthalate pH standard solution, phosphate pH standard equimolal
solution, and tetraborate pH standard solution) were purchased from
FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Trimethoxy(1H,1H,2H,2H-heptadecafluorodecyl)silane
(PFS) and fluorescent microbeads (Fluoresbrite YG Microspheres, Calibration
Grade 1.00 μm) were provided by Tokyo Chemical Industry Corporation
(Tokyo, Japan) and Polysciences, Inc. (Warrington, PA), respectively.
For PDMS fabrication, SILPOT 184 W/C purchased from Dow Toray Co.,
Ltd. was used. All materials were used without further purification.
(POPC) was purchased from NOF Corporation (Tokyo, Japan). Cholesterol
was provided by Avanti Polar Lipids. Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (triethylammonium salt)
(Texas Red DHPE) was supplied by Thermo Fisher Scientific, Inc. (Waltham,
MA). Glucose, sucrose, tris(hydroxymethyl)aminomethane (Tris), hydrochloric
acid, methanol, chloroform, hexadecane, liquid paraffin, and pH standard
solutions (phthalate pH standard solution, phosphate pH standard equimolal
solution, and tetraborate pH standard solution) were purchased from
FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Trimethoxy(1H,1H,2H,2H-heptadecafluorodecyl)silane
(PFS) and fluorescent microbeads (Fluoresbrite YG Microspheres, Calibration
Grade 1.00 μm) were provided by Tokyo Chemical Industry Corporation
(Tokyo, Japan) and Polysciences, Inc. (Warrington, PA), respectively.
For PDMS fabrication, SILPOT 184 W/C purchased from Dow Toray Co.,
Ltd. was used. All materials were used without further purification.
3
Biolipid-Polymer Nanoparticle Synthesis
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Poly-l -lysine (PLL, 150–300 kDa, 1 mg/mL aqueous solution), Polyethyleneimine (PEI, branched, 25 kDa by LS), In Vitro Toxicology Assay Kit resazurin-based, Dulbecco’s Modified Eagle’s Medium-high glucose with glutamine (DMEM), penicillin–streptomycin (P-S) and fetal bovine serum (FBS) were purchased from Sigma Aldrich (Sigma Aldrich, Algés, Portugal). 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) were purchased from Avanti Lipids (Alabaster, AL, USA). Texas Red™ 1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine Triethylammonium Salt (Texas Red-DHPE) was purchased from Thermo Fisher (Fisher Scientific Lda., Porto Salvo, Portugal). GFP plasmid (pCMV-GFP), obtained through Addgene, was a gift from Connie Cepko (Addgene plasmid #11153) [48 (link)]. The peptide employed for the synthesis of bPLL (CKKKKKC) were purchased at POP-UP, Faculty of Sciences, University of Porto (Porto, Portugal).
4
Lipid Reagents for Fluorescence Experiments
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1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (sodium salt) (POPG) were purchased from Avanti Polar Lipids (Alabaster, AL, USA) and NOF Corporation (Tokyo, Japan). Cholesterol was provided by Avanti Polar Lipids. Texas Red® 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (triethylammonium salt) (Texas Red DHPE) was supplied by Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Fructose, uranine, fluorescein, sodium hydroxide, hydrochloric acid, methanol, chloroform, and pH standard solutions (phthalate pH standard solution, phosphate pH standard equimolal solution, and tetraborate pH standard solution) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). 2,5-Bis(methylsulfonyl)-1,4-phenylenediamine (BMeS-p-A) and a solution (1 mM) of fluorescein-12-adenosine triphosphate (FL-ATP) with 10 mM Tris-HCl, pH 7.6, 1 mM EDTA were supplied by Tokyo Chemical Industry Corporation (Tokyo, Japan) and PerkinElmer, Inc. (Waltham, MA, USA), respectively. Fluorescent microbeads (Fluoresbrite® YG Microspheres, Calibration Grade 1.00 µm) were provided by Polysciences, Inc. (Warrington, PA, USA). All materials were used without further purification.
5
Lipid Mixture Preparation for Biophysical Studies
6
Phospholipid Preparation for Membrane Studies
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1-Palmitoyl-2-oleoyl-3-sn-glycero-3-phosphocholine (POPC) was purchased from Wako (Tokyo, Japan). 1-Stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′-bisphosphate) (18:0–20:4 PI(4,5)P2), 1,2-hexadecanoyl-3-sn-glycerophosphatidylinositol-(3,4,5)-trisphosphate (18:0–20:4 PI(3,4,5)P3), 1,2-dihexanoyl-sn-glycero-3-phospho-(1′-myo-inositol-4′,5′-bisphosphate) (08:0 PI(4,5)P2), and 1,2-dihexanoyl-sn-glycero-3-phospho-(1′-myo-inositol-3′,4′,5′-trisphosphate) (08:0 PI(3,4,5)P3) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). TexasRed®-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (TexasRed-DHPE) was provided by Invitrogen (Grand Island, NY, USA). Organic solvents and inorganic salts were provided by WAKO (Tokyo, Japan). These reagents were used as received.
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