Human renal cancer 786-O and ACHN cell lines were purchased from Bioresource Collection and Research Center (Hsinchu, Taiwan) and cultured in RPMI-1640 medium (Cytiva, SH30011.02) and MEM medium (Cytiva, SH30193.04). The human proximal tubule epithelial HK2 cell line was incubated with DMEM/F12 medium (Cytiva SH30004.03). These cell culture media contained 10% FBS (Cytiva SH30071.02), NEAA solution (100X; Cytiva SH30238.01), P/S reagent (100X, Cytiva SV30010) and cells incubated at 37 °C (involved in 5% CO2) in a humidified atmosphere. RCC and HK-2 cells were subcultured when cells reached 70% confluence.
Mem medium
Manufactured by Cytiva
Sourced in United States, China
MEM medium is a cell culture medium used to support the growth and maintenance of various cell types in vitro. It provides a balanced salt solution, amino acids, vitamins, and other essential nutrients required for cell proliferation and survival.
Automatically generated - may contain errors
Lab products found in correlation
Dmem f12 medium, by Cytiva (1 mentions)
Cisplatin, by Targetmol (1 mentions)
N acetyl l cysteine nac, by Merck Group (1 mentions)
Tert butyl hydroperoxide tbhp, by Maclin Biochemical Technology (1 mentions)
Lipo3000, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Cytiva (1 mentions)
Protease inhibitor mixture, by Thermo Fisher Scientific (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Corning (1 mentions)
4 protocols using mem medium
1
Culturing Renal Cell Lines
2
Modulating Redox Homeostasis in NSCLC
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Human NSCLC cell lines H1975, A427, and SKLU1 were cultured in RPMI-1640 medium (Cytiva) and A549 and H1299 in DMEM/HG medium (Cytiva). Human fetal lung fibroblast cell line MRC5 was cultured in MEM medium (Cytiva). 293T cells were cultured in a DMEM/HG medium. All culture medium contains 8% fetal bovine serum (FBS).
Plasmids overexpressing wild-type H1.2, N-terminal truncated H1.2 (H1.2-ΔN), NRF2, or GCLC, CRISPR/Cas9 plasmids for knocking out H1.2, and shRNAs targeting NRF2 or KEAP1 were constructed as described (49 (link)) (primers inSI Appendix, Table S3 ). Cells were transfected with indicated plasmids using Lipo3000 (Invitrogen). Tert-butyl hydroperoxide (tBHP) was from Macklin; tert-butyl hydroquinone (tBHQ), ML385, and cisplatin were from Targetmol; and N-acetyl-L-cysteine (NAC) was from Sigma.
Plasmids overexpressing wild-type H1.2, N-terminal truncated H1.2 (H1.2-ΔN), NRF2, or GCLC, CRISPR/Cas9 plasmids for knocking out H1.2, and shRNAs targeting NRF2 or KEAP1 were constructed as described (49 (link)) (primers in
3
Culturing Human Neuroblastoma TE671 Cells
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Human neuroblastoma TE671 cells were cultured in MEM medium (HyClone Laboratories, South Logan, UT, USA) supplemented with 10% fetal bovine serum, 100 U/mL penicillin, 100 μg/mL streptomycin, 2 mM glutamine and 1 mM sodium pyruvate at 37 °C in a humidified atmosphere of 5% CO2.
4
Activation of Natriuretic Peptide by sCorin
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HEK293 cells (ATCC, CRL‐1573, authenticated by short tandem repeat profiling) were cultured in DMEM (Corning) with 10% FBS. A plasmid expressing human pro‐ANP with a C‐terminal V5 tag31 was transfected into the cells using PolyJet reagents, as described above. To examine the activity of sCorin, human pro‐ANP in the conditioned medium from the transfected HEK293 cells was incubated with purified sCorin without or with enterokinase treatment. sCorin activation and pro‐ANP to ANP conversion were analyzed by western blotting. To verify the activity of sCorin–activated ANP, pro‐ANP without or with sCorin treatment was added to baby hamster kidney cells (NingBoMingZhou Tech, China) cultured in 96‐well plates with MEM medium (Hyclone Laboratories, Logan, UT) and 10% FBS. After 30 minutes at 37°C, the cells were lysed with 1% (v/v) Nonidet P‐40 in a solution with 5% (v/v) glycerol, 25 mmol/L Tris‐HCl (pH 7.4), 150 mmol/L NaCl, 1 mmol/L EDTA, and 2% (v/v) a protease inhibitor mixture (Thermo Fisher Scientific). Levels of cGMP in cell lysates were examined by ELISA (Enzo Life Sciences, Farmingdale, NY).
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