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5 methoxypsoralen

Manufactured by Merck Group
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Sourced in United States, Japan

5-Methoxypsoralen is a naturally occurring chemical compound. It functions as a photosensitizing agent, which means it can interact with ultraviolet light to produce a biological response.

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Lab products found in correlation

Penicillin, by Thomas Scientific (1 mentions) Streptomycin, by Thomas Scientific (1 mentions) Heat inactivated fetal calf serum, by Thomas Scientific (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Propidium iodide, by Wuhan Boster Biological Technology (1 mentions) Acridine orange, by Wuhan Boster Biological Technology (1 mentions) Sqstm1 p62, by Cell Signaling Technology (1 mentions) Phospho mtor ser2448, by Cell Signaling Technology (1 mentions) 3 methyladenine, by Merck Group (1 mentions) Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions) Aprotinin, by Merck Group (1 mentions) Leupeptin, by Merck Group (1 mentions) Sodium orthovanadate, by Merck Group (1 mentions) Ofloxacin, by Merck Group (1 mentions) Fenofibrate, by Fujifilm (1 mentions) Sodium lauryl sulfate, by Merck Group (1 mentions) Rose bengal, by Fujifilm (1 mentions)

3 protocols using 5 methoxypsoralen

1

Cytotoxicity Assay Protocol

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5-Methoxypsoralen (≥ 95% by HPLC), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), and dimethyl sulfoxide were obtained from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Acridine orange and propidium iodide were procured from Wuhan Boster Biological Technology Ltd. (Wuhan, China). Dulbecco’s modified Eagle’s medium and RPMI-1640 medium were obtained from Gibco Life Technologies (Grand Island, NY, USA). Heat-inactivated fetal calf serum, penicillin, and streptomycin were obtained from Thomas Scientific, High Hill Road, Swedesboro, U.S.A.
Guo H., He Y., Bu C, & Peng Z. (2019). Antitumor and apoptotic effects of 5-methoxypsoralen in U87MG human glioma cells and its effect on cell cycle, autophagy and PI3K/Akt signaling pathway. Archives of Medical Science : AMS, 15(6), 1530-1538.
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2

Autophagy and Apoptosis Regulation

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5-Methoxypsoralen or Bergapten (Bg), aprotinin, leupeptin, phenylmethylsulfonyl fluoride (PMFS), sodium orthovanadate, the known inhibitor of autophagy 3-Methyladenine (3MA) were purchased from Sigma Chemical (Milan, Italy). The specific caspases inhibitor Z-VAD-FMK (ZVF) was from R&D Systems (Milan, Italy). Antibodies used in this study were from Santa Cruz Biotechnology (Santa Cruz, CA) except SQSTM1/p62 and Phospho­mTOR (Ser2448) from Cell Signalling (Danvers, MA).
De Amicis F., Aquila S., Morelli C., Guido C., Santoro M., Perrotta I., Mauro L., Giordano F., Nigro A., Andò S, & Panno M.L. (2015). Bergapten drives autophagy through the up-regulation of PTEN expression in breast cancer cells. Molecular Cancer, 14, 130.
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3

Evaluation of Phototoxic Chemicals

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Twenty phototoxic chemicals and 10 non-phototoxic chemicals were evaluated, including 6 drugs, 8 antimicrobials, 6 fragrances, 6 UV absorbers, and 4 other chemicals. Amiodarone HCl (1), anthracene (7), chlorpromazine HCl (9), 5-methoxypsoralen (15), 8-methox-ypsoralen (16), 6-methylcoumarin (17), ofloxacin (18) , hexachlorophene (21), ethylhexyl dimethyl p-aminobenzoate (23), chlorhexidine dihydrochloride (24), sodium lauryl sulfate (27), p-aminobenzoic acid (28), sulisobenzone (29), and lactic acid (30) were purchased from Sigma-Aldrich Japan (Tokyo, Japan). Rose bengal (2), bithionol (4), 3,3'4,5 tetrachlorosalicylanilide (5), fenofibrate (6), benzophenone (11), ketoprofen (13), methyl n-methylantranilate ( 14), and norfloxacin (19) were purchased from Wako Pure Chemical Industries (Osaka, Japan). 3,4',5-Tribromosalicylanilide (3), musk ambrette (8), promethazine (10), musk xylene (12), protoporphyrin IX, disodium (20), 2-octyl salicylate ( 22), 2-octyl methacrylate (25), and penicillin G (26) were purchased from Tokyo Chemical Industry (Tokyo, Japan).
Toyoda A., Sugiyama M., Furihata S., Nishizumi K., Omori T, & Itagaki H. (2017). Development of a modified 3T3 Neutral Red Uptake Phototoxicity Test protocol for evaluation of poorly water-soluble substances. The Journal of toxicological sciences, 42(5).
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