Catalase cat activity assay kit

Manufactured by Sangon

Sourced in China

The Catalase (CAT) activity assay kit is a laboratory product that measures the enzymatic activity of catalase, an antioxidant enzyme found in living organisms. The kit provides a quantitative method to determine the catalase levels in various samples, such as cell lysates or tissue homogenates. The assay is based on the ability of catalase to decompose hydrogen peroxide (H2O2) into water and oxygen.

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2 protocols using catalase cat activity assay kit

Spinetoram Cytotoxicity Evaluation Protocol

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Spinetoram (CAS No: 187166-40-1, purity > 98%) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) and penicillin–streptomycin were obtained from Hyclone (Logan, UT, USA). Fetal bovine serum (FBS) was obtained from Gibco (Norristown, PA, USA). Phosphate buffered saline (PBS) was obtained from Servicebio (Wuhan, China).
The malondialdehyde (MDA) content assay kit (D799762-0100), reduced glutathione (GSH) content assay kit (D799614-0100), catalase (CAT) activity assay kit (D799598-9100), and superoxide dismutase (SOD) activity assay kit (D799594-0100) were bought from Sangon Biotech (Beijing, China). The LC3 antibody, Beclin1 antibody, p62 antibody, p-mTOR antibody, p-AMPK antibody, GAPDH antibody, and γH2AX antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). The Alexa fluor 488-conjugated antibody were purchased from Sangon Biotech (Shanghai, China).

Chen F., Lu J., Li M., Yang J., Xu W., Jiang X, & Zhang Y. (2024). Spinetoram-Induced Potential Neurotoxicity through Autophagy Mediated by Mitochondrial Damage. Molecules, 29(1), 253.

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Oxidative Stress Evaluation in Plants

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The procedures for nitroblue tetrazolium (NBT) and 3′‐diaminobenzidine (DAB) staining followed the description by Zhang et al. (2011 (link)). The electrolyte leakage rate, malondialdehyde (MDA) content and peroxidase (POD) activity were determined as described by Zang et al. (2017 (link)). The chlorophyll content was determined according to the method of Brennan and Frenkel (1977 (link)). H₂O₂ levels were measured using a Hydrogen Peroxide (H₂O₂) Content Assay Kit (Jiancheng Biotech, Nanjing, China). Ascorbate peroxidase (APX) and catalase (CAT) activity were measured using an APX Activity Assay Kit and Catalase (CAT) Activity Assay Kit (Micro method; Sangon Biotech Co., Ltd., Shanghai, China), respectively. Each sample contained at least 10 seedlings, and each experiment had three biological replicates.

Jia Y., Zhao H., Niu Y, & Wang Y. (2023). Long noncoding RNA from Betula platyphylla, BplncSIR1, confers salt tolerance by regulating BpNAC2 to mediate reactive oxygen species scavenging and stomatal movement. Plant Biotechnology Journal, 22(1), 48-65.

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