Mccoy 5a medium

The expression vector and PEG-COOH were from
our laboratory collection. Escherichia coli BLR(DE3) (Novagen) was used for protein production. Erdafitinib
was purchased from Wuhan Yongcan Biotechnology Co. Ltd (Wuhan, China).
Dimethyl sulfoxide (DMSO), calcein-AM, propidium iodide (PI), and
4% paraformaldehyde solution were bought from Aladdin (Shanghai, China).
The Cell Counting Kit-8 (CCK-8) was provided by APExBIO Technology.
Hematoxylin and Eosin (H&E), Ki67, trypsin–EDTA solution,
the Micro Blood Phosphorus Concentration Assay Kit, penicillin, and
streptomycin were purchased from Solarbio Science & Technology
(Beijing, China). The human bladder cancer cell line (T24 cells) was
bought from Keygen Biotechnology (Nanjing, China). The human bladder
cancer cell line (RT4 Cells) and McCoy’ 5A medium were purchased
from Procell life Science&Technology Co. Ltd. (Wuhan China). RPMI
1640 medium, phosphate-buffered saline (PBS), and fetal bovine serum
(FBS) were provided by Gibco life technologies.

The human EC cell lines Hec-1A (NTCC, Beijing, China), KLE (Procell, Wuhan, China) were obtained commercially, and Ishikawa was provided by the research group of Professor Ya Xie from the First Affiliated Hospital of Zhengzhou University. The use of the Ishikawa cell line had been approved by the Ethics Committee of the Third Affiliated Hospital of Zhengzhou University (Protocol # 2021-WZ-002). They were cultured in McCoy’5A medium (Procell, Wuhan, China) and DMEM medium (Solarbio, Beijing, China), respectively, which contained 10% serum, 100U/mL penicillin, and 100mg/mL streptomycin at 37°C in a humidified atmosphere containing 5% CO₂.