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Thunder imager 3d assay

Manufactured by Leica
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The THUNDER Imager 3D Assay is a high-performance imaging system designed for advanced 3D cell culture analysis. It provides accurate and reliable data acquisition and analysis for various 3D assays.

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Lab products found in correlation

Hcx pl apo fluotar 100 1.44 na oil immersion objective, by Leica (2 mentions) Scmos camera, by Leica (2 mentions) Las x premium software, by Leica (2 mentions) Ndp viewing software, by Hamamatsu Photonics (1 mentions) Nanozoomer 2.0 ht system, by Hamamatsu Photonics (1 mentions)

Close spelling variants of this product

THUNDER Imager Live Cell & 3D Cell Culture & 3D Assay Thunder Imager 3D Thunder Imager

3 protocols using thunder imager 3d assay

1

Quantitative Analysis of Amyloid Burden

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Images were obtained from an average of 3 sections per mouse for IHC and IF. For IHC stains, slides were scanned on the NanoZoomer 2.0-HT system (Hamamatsu Photonics) at 20x. Images were further processed using NDP viewing software (Hamamatsu Photonics) and Fiji software version 1.51 (National Institutes of Health). The sections were converted into 8-bit images followed by a selection of cerebral cortex and hippocampus using tissue landmarks. An appropriate threshold number (based on the preliminary analysis avoiding floor/ceiling effects) was applied consistently to highlight the majority of amyloid plaques ensuring no artifacts were incorporated in the quantification. Particles were analyzed and collated numbers were collected to calculate the mean amyloid burden between groups using GraphPad Prism software. For IF (Iba1 and GFAP) stains and slides were scanned on Leica Thunder imager 3D assay at 20x. Images were further processed using Fiji software as previously described.
Bosch M.E., Dodiya H.B., Michalkiewicz J., Lee C., Shaik S.M., Weigle I.Q., Zhang C., Osborn J., Nambiar A., Patel P., Parhizkar S., Zhang X., Laury M.L., Mondal P., Gomm A., Schipma M.J., Mallah D., Butovsky O., Chang E.B., Tanzi R.E., Gilbert J.A., Holtzman D.M, & Sisodia S.S. (2024). Sodium oligomannate alters gut microbiota, reduces cerebral amyloidosis and reactive microglia in a sex-specific manner. Molecular Neurodegeneration, 19, 18.
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2

Fluorescent In Situ Hybridization of Yeast Cells

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Cells were grown to mid-logarithmic phase and fixed with 4% formaldehyde in 0.1 M potassium phosphate buffer. Cells were then converted to spheroplasts using 0.1 M potassium phosphate buffer containing 1.2 M sorbitol and 500 μg of zymolyase. Spheroplasts were washed in 2× SSC buffer and incubated overnight at 37 °C with Cy3-labeled oligonucleotide probe (5′-Cy3-ATG CTC TTG CCA AAA CAA AAA AAT CCA TTT TCA AAA TTA TTA AAT TTC TT-3′) that is complementary to the 5′ portion of ITS1 (Faza et al., 2012). DNA was stained with 0.5 μg/ml DAPI. Cells were visualized using THUNDER Imager 3D Assay (Leica, Germany) equipped with a HCX PL-APO Fluotar 100×/1.44 NA oil immersion objective (Leica, Germany). Images were acquired with a fitted digital sCMOS camera and LAS X premium software (Leica, Germany).
Viéitez C., Busby B.P., Ochoa D., Mateus A., Memon D., Galardini M., Yildiz U., Trovato M., Jawed A., Geiger A.G., Oborská-Oplová M., Potel C.M., Vonesch S.C., Tu C.S., Shahraz M., Stein F., Steinmetz L.M., Panse V.G., Noh K.M., Savitski M.M., Typas A, & Beltrao P. (2021). High-throughput functional characterization of protein phosphorylation sites in yeast. Nature biotechnology, 40(3), 382-390.
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3

Fluorescent In Situ Hybridization of Yeast Cells

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Cells were grown to mid-logarithmic phase and fixed with 4% formaldehyde in 0.1 M potassium phosphate buffer. Cells were then converted to spheroplasts using 0.1 M potassium phosphate buffer containing 1.2 M sorbitol and 500 μg of zymolyase. Spheroplasts were washed in 2× SSC buffer and incubated overnight at 37 °C with Cy3-labeled oligonucleotide probe (5′-Cy3-ATG CTC TTG CCA AAA CAA AAA AAT CCA TTT TCA AAA TTA TTA AAT TTC TT-3′) that is complementary to the 5′ portion of ITS1 (Faza et al., 2012). DNA was stained with 0.5 μg/ml DAPI. Cells were visualized using THUNDER Imager 3D Assay (Leica, Germany) equipped with a HCX PL-APO Fluotar 100×/1.44 NA oil immersion objective (Leica, Germany). Images were acquired with a fitted digital sCMOS camera and LAS X premium software (Leica, Germany).
Viéitez C., Busby B.P., Ochoa D., Mateus A., Memon D., Galardini M., Yildiz U., Trovato M., Jawed A., Geiger A.G., Oborská-Oplová M., Potel C.M., Vonesch S.C., Tu C.S., Shahraz M., Stein F., Steinmetz L.M., Panse V.G., Noh K.M., Savitski M.M., Typas A, & Beltrao P. (2021). High-throughput functional characterization of protein phosphorylation sites in yeast. Nature biotechnology, 40(3), 382-390.
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