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Anti lrf

Manufactured by Thermo Fisher Scientific
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The Anti-LRF is a laboratory equipment product designed to detect and quantify the presence of the LRF (Leucine-Rich Repeat Flightless-Interacting Protein) in various biological samples. The core function of this product is to provide researchers with a tool to analyze and measure the levels of LRF, which is a protein involved in cellular processes. This equipment utilizes specialized techniques to accurately identify and measure the target analyte, without making any interpretations or extrapolations about its intended use.

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Lab products found in correlation

Mes sds running buffer, by Thermo Fisher Scientific (2 mentions) Anti β actin, by Santa Cruz Biotechnology (2 mentions) Anti ha, by Cell Signaling Technology (2 mentions) Anti bcl11a, by Abcam (2 mentions) Anti nfic, by Cell Signaling Technology (2 mentions) Anti nfia, by Active Motif (2 mentions) Anti nfix clone 3d2, by Merck Group (2 mentions) Anti hemoglobin γ, by Santa Cruz Biotechnology (2 mentions)

2 protocols using anti lrf

1

Immunoblotting of Differentiated HUDEP2 Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting of proteins were performed by following standard protocol. Briefly, total protein from HUDEP2 cells at day 5 of differentiation or primary cells at day 13 of differentiation were extracted with RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA)58 (link). Equivalent amounts of protein (10-20 µg) were loaded onto 4-12% or 10% Bis-Tris protein gels (Invitrogen) and separated in 1× MES-SDS running buffer (Invitrogen, NP0002). Separated proteins were transferred onto PVDF membrane and incubated with primary antibodies overnight. Primary antibodies used were anti-NFIA (Active Motif, #39397), 1:5,000; anti-NFIX (clone 3D2, Sigma Aldrich, SAB1401263), 1:500; anti-NFIC (Cell Signaling, #11911), 1:1,000; anti-BCL11A (Abcam, ab19487), 1:1,000; anti-LRF (Thermo Fisher Scientific, #14-3309-82), 1:2,000; anti-hemoglobin γ (Santa Cruz, #21756), 1:1,000; anti-β-Actin (Santa Cruz, #47778), 1:1,000; anti-HA (Cell Signaling, #3724), 1:1,000.
Qin K., Huang P., Feng R., Keller C.A., Peslak S.A., Khandros E., Saari M., Lan X., Mayuranathan T., Doerfler P.A., Abdulmalik O., Giardine B., Chou S.T., Shi J., Hardison R.C., Weiss M.J, & Blobel G.A. (2022). Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells. Nature genetics, 54(6), 874-884.
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2

Immunoblotting of Differentiated HUDEP2 Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting of proteins were performed by following standard protocol. Briefly, total protein from HUDEP2 cells at day 5 of differentiation or primary cells at day 13 of differentiation were extracted with RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA)58 (link). Equivalent amounts of protein (10-20 µg) were loaded onto 4-12% or 10% Bis-Tris protein gels (Invitrogen) and separated in 1× MES-SDS running buffer (Invitrogen, NP0002). Separated proteins were transferred onto PVDF membrane and incubated with primary antibodies overnight. Primary antibodies used were anti-NFIA (Active Motif, #39397), 1:5,000; anti-NFIX (clone 3D2, Sigma Aldrich, SAB1401263), 1:500; anti-NFIC (Cell Signaling, #11911), 1:1,000; anti-BCL11A (Abcam, ab19487), 1:1,000; anti-LRF (Thermo Fisher Scientific, #14-3309-82), 1:2,000; anti-hemoglobin γ (Santa Cruz, #21756), 1:1,000; anti-β-Actin (Santa Cruz, #47778), 1:1,000; anti-HA (Cell Signaling, #3724), 1:1,000.
Qin K., Huang P., Feng R., Keller C.A., Peslak S.A., Khandros E., Saari M., Lan X., Mayuranathan T., Doerfler P.A., Abdulmalik O., Giardine B., Chou S.T., Shi J., Hardison R.C., Weiss M.J, & Blobel G.A. (2022). Dual function NFI factors control fetal hemoglobin silencing in adult erythroid cells. Nature genetics, 54(6), 874-884.
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