Primers uu1613f and uu1524r

Manufactured by Thermo Fisher Scientific

Primers UU1613F and UU1524R are oligonucleotides designed for use in molecular biology applications. The UU1613F primer is 20 nucleotides long, and the UU1524R primer is 19 nucleotides long. These primers can be used in various DNA amplification techniques, such as PCR (Polymerase Chain Reaction), to target specific genetic sequences.

Automatically generated - may contain errors

Lab products found in correlation

Taqman fast advanced master mix, by Thermo Fisher Scientific (2 mentions) Nuclease free water, by Thermo Fisher Scientific (2 mentions) Viia7 real time pcr thermocycler, by Thermo Fisher Scientific (2 mentions) Uu parvo fam and uu t960 vic, by Thermo Fisher Scientific (1 mentions)

2 protocols using primers uu1613f and uu1524r

Real-time PCR Assay for Ureaplasma Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

For detection of Ureaplasma sp. DNA, amniotic fluid DNA was analysed using a real-time PCR assay targeting the urease gene of U. parvum and U. urealyticum as described by Yi et al.
[29 (link)], adapted for use on a ViiA7 real-time PCR thermocycler (Life Technologies, Carlsbad, California, USA). Reaction mixtures (final volume or concentration) consisted of: 1X Taqman FAST Advanced Master Mix (Life Technologies), 0.9 μM primers UU1613F and UU1524R (Life Technologies), 0.25 μM probes UU-parvo (FAM) and UU-T960 (VIC) (Life Technologies), 5 μL of template DNA and nuclease-free water (Ambion) to a final volume of 20 μL. PCR cycling conditions consisted of an initial denaturation/Taq activation at 95°C for 20 s, followed by 40 quantification cycles of 95°C for 1 s and 60°C for 20 s (data acquiring).

Payne M.S., Feng Z., Li S., Doherty D.A., Xu B., Li J., Liu L., Keelan J.A., Zhou Y.H., Dickinson J.E., Hu Y, & Newnham J.P. (2014). Second trimester amniotic fluid cytokine concentrations, Ureaplasma sp. colonisation status and sexual activity as predictors of preterm birth in Chinese and Australian women. BMC Pregnancy and Childbirth, 14, 340.

+ Open protocol

+ Expand

Quantitative Detection of Ureaplasma spp.

Check if the same lab product or an alternative is used in the 5 most similar protocols

Payne et al. [25] had already established species-level identification of UK triple-cloned isolates. For the Australian isolates, to determine whether the triple-cloned Ureaplasma spp. were either U. parvum or U. urealyticum, a real-time PCR assay targeting the urease gene of these species as described by Yi et al. [29] , adapted for use on a ViiA7 real-time PCR thermocycler (Life Technologies, Carlsbad, California, USA), was used. Reaction mixtures (final volume or concentration) consisted of: 1X Taqman FAST Advanced Master Mix (Life Technologies), 0.9 µM primers UU1613F and UU1524R (Life Technologies), 0.25 µM probes UU-parvo (FAM) and UU-T960 (VIC) (Life Technologies), 5 µL of template DNA and nuclease-free water (Ambion) to a final volume of 20 µL. PCR cycling conditions consisted of an initial denaturation/Taq activation at 95 o C for 20 s, followed by 40 quantification cycles of 95°C for 1 s and 60°C for 20 s (data acquiring).

Furfaro L.L., Spiller O.B., Keelan J.A, & Payne M.S. (2015). In vitro activity of solithromycin and its metabolites, CEM-214 and N-acetyl-CEM-101, against 100 clinical Ureaplasma spp. isolates compared with azithromycin. International journal of antimicrobial agents, 46(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!