10,000 Vero E6 cells per well were seeded into 24-well plates, treated with DMSO or MTX and infected with SARS-CoV-2 as indicated. After 48 h, the cells were lysed with the Lysis Binding Buffer from the Magnapure LC Kit 03038505001 (Roche) containing > 3 M guanidine thiocyanate (GTC), and ATP levels were quantified by adding luciferase as well as luciferin to a 1 µl sample of the lysate, using 100 µl of the CellTiter-Glo® Luminescent Cell Viability Assay solution (Promega), followed by luminometry using a Centro LB 960 luminometer (Berthold).
Magnapure lc kit
Manufactured by Roche
Sourced in Germany
The Magnapure LC Kit is a laboratory equipment product designed for nucleic acid purification. It utilizes magnetic bead technology to facilitate the extraction and purification of DNA or RNA samples. The kit provides a streamlined workflow and is suitable for use in various applications that require high-quality nucleic acid preparations.
Automatically generated - may contain errors
Lab products found in correlation
Centro lb 960 luminometer, by Berthold Technologies (1 mentions)
Celltiter glo luminescent cell viability assay solution, by Promega (1 mentions)
Nextseq 500 system, by Illumina (1 mentions)
Nextera xt dna library prep kit, by Illumina (1 mentions)
Superscript 4 one step rt pcr system, by Thermo Fisher Scientific (1 mentions)
3 protocols using magnapure lc kit
1
SARS-CoV-2 Infection Assay in Vero E6 Cells
2
Rapid RSV Genome Sequencing from Nasal Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Nucleic acids were extracted from RSV-positive nasal specimens using the MagNA Pure LC kit (Roche Diagnostics, Mannheim, Germany) as previously described (18 (link)). RSV subtyping and quantification were performed by multiplexed TaqMan RT-PCR analysis of the RSV N gene using RSV A and RSV B specific primer/probe mixes. Subsequently, subtype-specific RT-PCR was performed using the SuperScript IV one-step RT-PCR system (Invitrogen, Carlsbad, CA USA) to amplify 4 overlapping fragments covering the full RSV genome. The resultant 3.5 to 5.0 kb amplicons were pooled, purified from 1% agarose gels, used to construct libraries by means of the Nextera XT DNA Library Prep kit, and sequenced on a NextSeq 500 system (Illumina, San Diego, CA USA) (18 (link)).
3
SARS-CoV-2 RNA Isolation and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For RNA isolation, the SARS-CoV-2 containing cell culture supernatant was mixed (1:1 ratio) with the Lysis Binding Buffer from the Magnapure LC Kit # 03038505001 (Roche) to inactivate the virus. The viral RNA was isolated using Trizol LS, chloroform, and isopropanol. After washing the RNA pellet with ethanol, the isolated RNA was resuspended in nuclease-free water. Quantitative RT-PCR was performed according to a previously established RT-PCR assay involving a TaqMan probe (Corman et al., 2020) , to quantify virus yield. The following oligonucleotides were used for qRT-PCR, which amplify a genomic region corresponding to the envelope protein gene (26,141 -26,253), as described (Corman et al., 2020) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!