The SP concentrations of the AH samples were tested by the Human SP ELISA kits (ab133029, Abcam, MA, USA). Specifically, 50 μl AH sample or standards were added to the wells, along with an alkaline phosphatase (AP) conjugated-SP antigen and a polyclonal rabbit antibody specific to SP. The plate was incubated at room temperature for 2 h, then after a wash, the pNpp substrate was added. After a incubation of 1 h at room temperature, stop solution was added and the plate was read immediately at 405 nm. The optical density was inversely proportional to the amount of SP captured in the plate.
Human sp elisa kits
Manufactured by Abcam
Sourced in United States
The Human SP ELISA kits are quantitative sandwich enzyme-linked immunosorbent assay (ELISA) kits designed for the measurement of human Substance P levels in biological samples such as serum, plasma, and cell culture supernatants.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using human sp elisa kits
1
Quantifying Substance P in Aqueous Humor
2
Aqueous Humor SP Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The SP concentrations of the AH samples were tested by the Human SP ELISA kits (ab133029, Abcam, MA, USA). Specifically, 50 µl AH sample or standards were added to the wells, along with an alkaline phosphatase (AP) conjugated-SP antigen and a polyclonal rabbit antibody specific to SP. The plate was incubated at room temperature for 2 hours, then after a wash, the pNpp substrate was added. After a incubation of 1 hour at room temperature, stop solution was added and the plate was read immediately at 405 nm. The optical density was inversely proportional to the amount of SP captured in the plate.
Statistical Analysis: SPSS software version 20.0 (SPSS Inc., Chicago, IL) was used. Comparisons of both eyes of the same patients utilized the paired t test to determine if the data is of normal distribution, or otherwise the Wilcoxon signed rank test was used with the Bonferroni correction. Comparisons of the differences (the second surgical eye minus the first one) between the ARC patients and diabetic patients were conducted using the independent t test to determine if the data is of normal distribution, or otherwise the Mann-Whitney U rank test was utilized. Two-tailed P < 0.05 were considered to indicate a statistically significant difference.
Statistical Analysis: SPSS software version 20.0 (SPSS Inc., Chicago, IL) was used. Comparisons of both eyes of the same patients utilized the paired t test to determine if the data is of normal distribution, or otherwise the Wilcoxon signed rank test was used with the Bonferroni correction. Comparisons of the differences (the second surgical eye minus the first one) between the ARC patients and diabetic patients were conducted using the independent t test to determine if the data is of normal distribution, or otherwise the Mann-Whitney U rank test was utilized. Two-tailed P < 0.05 were considered to indicate a statistically significant difference.
3
Aqueous Humor SP Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SP concentrations of the AH samples were tested by the Human SP ELISA kits (ab133029, Abcam, MA, USA). Specifically, 50 µl AH sample or standards were added to the wells, along with an alkaline phosphatase (AP) conjugated-SP antigen and a polyclonal rabbit antibody specific to SP. The plate was incubated at room temperature for 2 hours, then after a wash, the pNpp substrate was added. After a incubation of 1 hour at room temperature, stop solution was added and the plate was read immediately at 405 nm. The optical density was inversely proportional to the amount of SP captured in the plate.
Statistical Analysis: SPSS software version 20.0 (SPSS Inc., Chicago, IL) was used. Comparisons of both eyes of the same patients utilized the paired t test to determine if the data is of normal distribution, or otherwise the Wilcoxon signed rank test was used with the Bonferroni correction. Comparisons of the differences (the second surgical eye minus the first one) between the ARC patients and diabetic patients were conducted using the independent t test to determine if the data is of normal distribution, or otherwise the Mann-Whitney U rank test was utilized. Two-tailed P < 0.05 were considered to indicate a statistically significant difference.
Statistical Analysis: SPSS software version 20.0 (SPSS Inc., Chicago, IL) was used. Comparisons of both eyes of the same patients utilized the paired t test to determine if the data is of normal distribution, or otherwise the Wilcoxon signed rank test was used with the Bonferroni correction. Comparisons of the differences (the second surgical eye minus the first one) between the ARC patients and diabetic patients were conducted using the independent t test to determine if the data is of normal distribution, or otherwise the Mann-Whitney U rank test was utilized. Two-tailed P < 0.05 were considered to indicate a statistically significant difference.
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