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Protease inhibitor cocktail 111

Manufactured by Merck Group
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Protease inhibitor cocktail 111 is a laboratory reagent designed to inhibit the activity of proteases, which are enzymes that break down proteins. The cocktail contains a specific combination of chemical compounds that can effectively suppress the function of a variety of proteases, making it a useful tool for researchers working with protein samples.

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Lab products found in correlation

Phosphatase inhibitor cocktail 1 and 11, by Merck Group (2 mentions)

Close spelling variants of this product

Protease inhibitor cocktail Proteases inhibitor cocktail Protease cocktails Cocktail inhibitor Protease inhibitors

2 protocols using protease inhibitor cocktail 111

1

PPM1G Phosphorylation Assay in Glioma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glioma cells were pretreated, as indicated, with vehicle, LY294002, or MK-2206 for 1 hour followed by incubation in phosphate-free media containing vehicle/inhibitor(s) for 30 minutes. 200μCi 32P-orthophosphate/ml was then added for 2 hours. After labeling, cells were harvested/lysed in buffer containing 50mM Tris-Cl (pH 7.4), 150mM NaCl, 1mM EDTA, 1% Triton X-100, protease inhibitor cocktail 111 (Sigma), and phosphatase inhibitor cocktail 1 and 11 (Sigma). Immunoprecipitation with anti-PPM1G or anti-FLAG antibody was then performed, resolved by SDS-PAGE and electrotransferred to PVDF membrane. 32P-labeling of PPM1G was detected by phosphorimaging as described above. Immunoblotting was then performed using antibodies against PPM1G by standard procedures.
Xu K., Wang L., Feng W., Feng Y, & Shu H.K. (2016). Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase. Oncogene, 35(44), 5807-5816.
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2

PPM1G Phosphorylation Assay in Glioma Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Glioma cells were pretreated, as indicated, with vehicle, LY294002, or MK-2206 for 1 hour followed by incubation in phosphate-free media containing vehicle/inhibitor(s) for 30 minutes. 200μCi 32P-orthophosphate/ml was then added for 2 hours. After labeling, cells were harvested/lysed in buffer containing 50mM Tris-Cl (pH 7.4), 150mM NaCl, 1mM EDTA, 1% Triton X-100, protease inhibitor cocktail 111 (Sigma), and phosphatase inhibitor cocktail 1 and 11 (Sigma). Immunoprecipitation with anti-PPM1G or anti-FLAG antibody was then performed, resolved by SDS-PAGE and electrotransferred to PVDF membrane. 32P-labeling of PPM1G was detected by phosphorimaging as described above. Immunoblotting was then performed using antibodies against PPM1G by standard procedures.
Xu K., Wang L., Feng W., Feng Y, & Shu H.K. (2016). Phosphatidylinositol-3 kinase-dependent translational regulation of Id1 involves the PPM1G phosphatase. Oncogene, 35(44), 5807-5816.
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