Striatum was homogenized in RIPA buffer containing protease and phosphatase inhibitors (1 mM sodium orthovanadate, 1 mM aprotinin, and 1% protease inhibitor cocktail) and centrifuged at 10000g for 10 minutes at 4°C. Supernatants were used for western blotting, as described in da Rosa-Junior.33 (link) The following primary antibodies were used: anti-phospho-JNK and anti-JNK were purchased from R&D Systems, Minnesota; anti-Bok, anti-Bcl-xL, anti-caspase-9, anti-caspase-3, anti-cleaved caspase-3, anti-phospho-p38, anti-p38, anti-phospho-ERK1/2, and anti-ERK1/2 from Cell Signaling, Massachusetts; anti-superoxide dismutase 1 (SOD1), anti-heme oxygenase-1 (HO-1), and anti-α-synuclein from Abcam, Cambridge, UK; anti-catalase (CAT) was from Merk Millipore, Massachusetts; anti-GSK-3β from Santa Cruz Biotechnology, Texas; anti-β-actin from Sigma-Aldrich, Missouri.
Anti phospho jnk
Manufactured by R&D Systems
Sourced in United States
Anti-phospho-JNK is a primary antibody that specifically recognizes the phosphorylated form of the c-Jun N-terminal kinase (JNK) protein. JNK is a member of the mitogen-activated protein kinase (MAPK) family and plays a key role in cellular stress response pathways. This antibody can be used to detect and quantify the levels of phosphorylated JNK in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti β actin, by Merck Group (2 mentions)
Anti total jnk, by R&D Systems (2 mentions)
Anti gsk3β, by Santa Cruz Biotechnology (1 mentions)
Anti bok, by R&D Systems (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti α synuclein, by Abcam (1 mentions)
Anti jnk, by R&D Systems (1 mentions)
Anti phospho erk1 2, by Cell Signaling Technology (1 mentions)
Anti bcl xl, by Cell Signaling Technology (1 mentions)
Anti erk1 2, by Cell Signaling Technology (1 mentions)
Anti p38, by Cell Signaling Technology (1 mentions)
Anti phospho p38, by Cell Signaling Technology (1 mentions)
Anti caspase 9, by Cell Signaling Technology (1 mentions)
Anti caspase 3, by Cell Signaling Technology (1 mentions)
Chrysin, by Merck Group (1 mentions)
Dimethyl sulphoxide dmso, by Merck Group (1 mentions)
Anti phospho p38, by R&D Systems (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Anti nf κb p65, by Thermo Fisher Scientific (1 mentions)
Anti gapdh antibody, by Santa Cruz Biotechnology (1 mentions)
3 protocols using anti phospho jnk
1
Striatum Protein Analysis by Western Blot
2
Doxorubicin-Induced Oxidative Stress Modulation
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DOX was purchased as Adriablastine (50 mg doxorubicin hydrochloride, Pharmacia & Upjohn, Milan, Italy). Chrysin, GSH, Ellman’s reagent [5,5-dithio-bis (2-nitrobenzoic acid); DTNB], bovine serum albumin, dimethylsulphoxide (DMSO) and thiobarbituric acid were purchased from Sigma Chemical Co. (St Louis, MO, USA). Anti total p38, anti phospho p38, anti total JNK and anti phospho JNK antibodies were purchased from R & D systems (Minneapolis, USA). Anti total AKT and anti phospho AKT antibodies were purchased from Biovision Co. (San Francisco, USA). Anti GAPDH antibody was purchased from Santa cruz biotechnology (Santa cruz, Texas, USA). Anti NF-κB p65, VEGF, p53, cytochrome c, caspase 3 antibodies were purchased from Thermo Fisher Scientific Co. (Waltham, MA, USA). All other chemicals were of the highest purity grade commercially available.
3
Molecular Mechanisms of Cell Signaling
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If not stated differently chemicals and reagents were purchased from Sigma--Aldrich (Missouri, USA). All cell culture reagents were purchased from Life Technologies (Zug, Switzerland). The following antibodies were used: Anti HIF--1α (NOVUS, Littleton, USA), anti Rac1, anti β--Actin (Sigma), anti TUJ--1 (COVANCE, Berkeley, CA, USA), anti phospho--GSK3β and anti total GSK3α/β (Millipore, Billerica, MA, USA), anti phospho--JNK and anti total JNK (R&D Systems, Minneapolis, MN, USA), anti phospho--p55 and p85 PI3K and total p85 PI3K, anti phospho--AKT and anti total AKT (Cell signaling, Danvers, MA, USA).
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