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Sanguinarine

Manufactured by Bio-Techne

Sanguinarine is a laboratory reagent produced by Bio-Techne. It is a naturally occurring benzophenanthridine alkaloid. Sanguinarine exhibits fluorescent properties and can be used for various research applications.

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2 protocols using sanguinarine

1

Synthesis and Purification of Bioactive Compounds

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The synthesis of Hipp has been previously described (Somaiah et al. 2014 (link)). Elatol was synthesized as previously described and was a kind gift from Tyler Fulton, Carina Jette, and Dr. Brian Stoltz (Caltech) (White et al. 2008 (link)). Sanguinarine and 15d-Δ12,14-prostaglandin-J2 were purchased from Tocris Bioscience and Enzo Life Sciences, respectively. Elisabatin A was purified from the West Indian sea whip as previously described (Rodríguez et al. 1999 (link)). 6-α-aminocholestanol was custom synthesized by Toronto Research Chemicals. All compounds were resuspended in DMSO, with the exception of 6-α-aminocholestanol which was in methanol. All stocks were stored at −80°C.
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2

Signaling Pathway Inhibitors and Antibodies

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U0126 was purchased from VWR International (West Chester, PA), JC-1 from Invitrogen (Grand Island, NY), Sanguinarine from Tocris Biosciences (Minneapolis, MN), and Lapatinib from Selleckchem (Houston, TX). Antibodies for MKP1, ERK, pERK, JNK, pJNK, HER2, and TOM40 were purchased from Sigma (St. Louis, MO); COXIV and pMKP1 were purchased from Cell Signaling (Beverly, MI).
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