Ht 29 human crc

The HCT-116 and HT-29 human CRC cell lines were purchased from the ATCC (Manassas, VA, USA). RPMI 1640 medium (Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin was used to culture both kinds of cells. Cells were cultured in a 37 °C incubator with 5% CO₂. Sodium butyrate (NaB) was purchased from Aladdin (Shanghai, China). Recombinant human IL-6 protein was obtained from R&D Systems (Minneapolis, MN, USA).

Caco-2 human colon adenocarcinoma, NCI-H1975 human non-small cell lung cancer (NSCLC), 786-O human RCC, HT-29 human CRC, LoVo human CRC, SW480 human CRC, NCI-H292 human pulmonary mucoepidermoid carcinoma, NCI-H69 human SCLC, 4T1 mouse breast cancer, and MX-1 human triple negative breast cancer cells were acquired from ATCC and cultured according to the provider’s instructions. LLC-PK1 porcine renal epithelial cells overexpressing human P-gp (L-MDR1 cells) were obtained from Prof. Schinkel at Netherlands Cancer Institute (Amsterdam, The Netherlands). The recombinant NCI-H1975-P-gp and NCI-H1975-BCRP NSCLC cell lines were generated by the Natural and Medical Sciences Institute (NMI; University of Tuebingen, Tuebingen, Germany) by lentiviral transduction using the plasmids pGT414_Hyg_PGP and pGT414_Hyg_BCRP. The cancer cell lines were authenticated using short tandem repeat DNA fingerprinting at DSMZ and subjected frequently to mycoplasma testing.