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G6p assay kit with wst 8

Manufactured by Beyotime
Sourced in China

The G6P Assay Kit with WST-8 is a product designed for the quantitative determination of glucose-6-phosphate (G6P) in various samples. The kit utilizes the WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) reagent to facilitate the colorimetric detection of G6P.

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3 protocols using g6p assay kit with wst 8

1

Quantifying Cellular G6P Levels

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The level of G6P in cells was evaluated using a G6P Assay Kit with WST-8 (Beyotime, Shanghai, China) per manufacturer’s instructions.
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2

Glucose-Induced G6P Quantification

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Islets treated with 2.8 mM or 16.7 mM glucose were lysed and G6P level in the lysate was determined using G6P Assay Kit with WST-8 (Cat#S0185, Beyotime).
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3

Measuring Cellular Metabolic Profiles

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The glucose uptake rate, extracellular lactate secretion rate, glucose‐6‐phosphate (G6P) and G6PD activities in individual cell samples were analyzed using the glucose colorimetric assay kit II (Biovision), lactate colorimetric assay kit II (Biovision), G6PDH activity assay kit with WST‐8 (Beyotime), and G6P assay kit with WST‐8 (Beyotime) according to the manufacturer's instructions.
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