Rat tnf α elisa kit

Trinitrobenzene sulfonic (lot: SLCD2161) was purchased from Sigma (St. Louis, MO, United States). Mesalazine (lot: 14055) was purchased from Shanghai Haoyuan Chemexpress Co., Ltd (Shanghai, China). The rat TNF-α ELISA Kit (lot: 20200930) was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). A BioTek luciferase chemiluminescence detector (Winooski, VT, United States) was also used.

Main reagents and instruments included Rat IL-1β ELISA Kit (ML037361, China); Il-6 RAT ELISA Kit (ML102828, China); Rat TNF-α ELISA Kit (Ml002859, China); Rat COX-2 ELISA Kit (Ml058808, China); Rat PGE2 ELISA Kit (ML003036, China); MDA Assay Kit (A003-1-2, Nanjing Jiancheng, China); SOD Assay Kit (A001-3-2, Nanjing Jiancheng, China); CAT Assay Kit (A007-1-1, Nanjing Jiancheng, China); Rat IgG ELISA Kit (ML003019, China); Rat SIgA ELISA Kit (Ml003136, China); NGF antibody (DF6061, affinity, USA); carrageenan (C1013-25G, Merck, USA); electric constant temperature blast drying oven (DGG-9140B, Shanghai Shenxin, China); high-speed refrigerated centrifuge (Thermo Scientific, USA); microplate reader (SPECTCA MAX190, Molecular, USA); fast mixer (MX-S, SCILOGEX, USA); upright fluorescence microscope (NIKON ECLIPSE C1, Nikon, Japan).