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Renilla expression vector prl tk

Manufactured by Promega
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The Renilla expression vector pRL-TK is a plasmid designed for the expression of the Renilla luciferase gene in mammalian cells. It contains the Renilla luciferase coding sequence under the control of the herpes simplex virus thymidine kinase (HSV-TK) promoter, which provides constitutive expression in a wide range of cell types.

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Lab products found in correlation

Dual glo luciferase assay system, by Promega (2 mentions) Pmir report vector, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Renilla luciferase expression vector pRL-TK PRL-TK Renilla vector PRL-TK Renilla PRL-TK vector PRL-TK Renilla-TK

2 protocols using renilla expression vector prl tk

1

Cloning and Luciferase Assay of Cdc40 3'‐UTR

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The 3′‐UTR of Cdc40 sequences was amplified and cloned into a pMIR‐Report vector (Thermo Fisher Scientific) located downstream of the luciferase gene. The same amount of the Renilla expression vector pRL‐TK (Promega, Madison, WI, USA) was cotransfected for normalization. Luciferase activity was measured at 48 hours post‐transfection using the Dual‐Glo Luciferase Assay System (Promega).
Zhu Y., Qiao L., Zhou Y., Ma N., Wang C, & Zhou J. (2018). Long non‐coding RNA FOXD2‐AS1 contributes to colorectal cancer proliferation through its interaction with microRNA‐185‐5p. Cancer Science, 109(7), 2235-2242.
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2

Luciferase Assay for miR-370 and FOXM1 Interaction

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The 293T cells were co-transfected with a bioluminescent reporter vector (pMIR-REPORT-3′ UTR-FOXM1-wt/-mut or pMIR-REPORT-circRNA-wt/-mut) and the miR-370 ectopic expression vector. Equivalent amounts of Renilla expression vector pRLTK (Promega, Wisconsin, USA) were co-transfected into every group for normalization. After 48 hr, cells were harvested, and luciferase activities were measured using the Dual-Glo Luciferase Assay System (Promega, Fitchburg, WI, USA).
Chen Q., Zhang J., He Y, & Wang Y. (2018). hsa_circ_0061140 Knockdown Reverses FOXM1-Mediated Cell Growth and Metastasis in Ovarian Cancer through miR-370 Sponge Activity. Molecular Therapy. Nucleic Acids, 13, 55-63.
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