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Methotrexate

Manufactured by Targetmol
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Methotrexate is a synthetic folic acid analogue used as an active pharmaceutical ingredient in various laboratory applications. It functions as an enzyme inhibitor, primarily targeting the dihydrofolate reductase enzyme. Methotrexate is a widely utilized tool in biochemical research and analysis.

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Lab products found in correlation

Sb203580, by Targetmol (1 mentions) M6a antibody, by Thermo Fisher Scientific (1 mentions) Rabbit igg, by Merck Group (1 mentions) P38 mapk, by Cell Signaling Technology (1 mentions) Igf2bp1, by Cell Signaling Technology (1 mentions) Phospho p38 mapk, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Myc tag, by Immunoway (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)

2 protocols using methotrexate

1

Western Blot Analysis of Protein Targets

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Antibodies against the following proteins were used for Western blot: RPS15 (1:2000, Abcam; ab157193), IGF2BP1 (1:1000, Cell Signaling Technology; 8482), phospho-p38 MAPK (1:1000, Cell Signaling Technology; 9211), p38 MAPK (1:1000, Cell Signaling Technology; 9218), MKK6 (1:1000, Cell Signaling Technology; 8550), β-actin (1:1000, Cell Signaling Technology; 4970), Flag-tag (1:5000, Immunoway; YM3001), Myc-tag (1:5000, Immunoway; YM3203), m6A antibody (Thermo; MA5-33030) and rabbit IgG (Millipore; PP64B)-Diquafosol tetrasodium (CAS: 211427-08-6; T7423), Coenzyme A (CAS: 85-61-0; T10857), Folic acid (CAS: 59-30-3; T0062), Epicatechin gallate (CAS: 1257-08-5; T2732), and Methotrexate (CAS: 59-05-2; T1485) were purchased from TargetMol and SB203580 (CAS: 152121-47-6; T1764).
Zhao Y., Li Y., Zhu R., Feng R., Cui H., Yu X., Huang F., Zhang R., Chen X., Li L., Chen Y., Liu Y., Wang J., Du G, & Liu Z. (2023). RPS15 interacted with IGF2BP1 to promote esophageal squamous cell carcinoma development via recognizing m6A modification. Signal Transduction and Targeted Therapy, 8, 224.
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2

Methotrexate and Valsartan Dissolution Protocol

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Methotrexate and Valsartan were purchased from TargetMol, Massachusetts, United States (MolPort-003-665-521 and MolPort-003-666-608 respectively) and dissolved in DMSO (dimethyl sulfoxide; Millipore, Molsheim, France).
Kutbi D, & Almalki R.S. (2024). Valsartan Mitigates the Progression of Methotrexate-Induced Acute Kidney Injury in Rats via the Attenuation of Renal Inflammation and Oxidative Stress. Journal of Inflammation Research, 17, 2233-2243.
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