Goat anti rat fitc

Manufactured by Southern Biotech

Goat anti-rat-FITC is a fluorescently labeled secondary antibody that binds to rat primary antibodies. It is used for detection and visualization of rat-specific targets in various biological applications.

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Lab products found in correlation

Annexin 5 bv421, by BD (1 mentions) Macsquant analyzer 10, by Miltenyi Biotec (1 mentions) Anti cd4 apc cy7, by BD (1 mentions) Collagenase cls4, by Worthington (1 mentions) Anti cd3 v450, by BD (1 mentions)

Close spelling variants of this product

Goat anti-rat-IgG-FITC (2 citations) Goat anti-rat-FITC secondary antibody (2 citations)

2 protocols using goat anti rat fitc

Cell Death and Cell Cycle Analysis

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For cell death analyses, cells were incubated 30 min at RT with BV421 AnnexinV (563973, BD Biosciences, San Jose, CA) (dilution 1/10) and then incubated for 30 min at RT with 100 μgml−1 RNAse A and 25 μgml−1 propidium iodide. A maximum of 10,000 events were counted per condition using MACSquant Analyzer 10 (Miltenyi) and analysed with FlowJo10.
For cell cycle analyses, cultured cells were treated for 10 min with 30 μM BrdU (AlfaAesar #H27260–06), trypsinized, centrifuged and resuspend in 500μl PBS. Drop by drop 1.5ml ice-cold 100% ethanol was added to the cells. Pepsin (Sigma #p7000) (20min at 37degree) and HCL (20 min at room temperature) treatment were performed before labelling with Rat-anti-BrdU (AbD serotec #OBT0030S), Goat anti-rat-FITC (Southern Biotech #3030–02) and propidium iodide (AlfaAesar #J667664-MC). Flow cytometry was performed using MACSquant Analyzer 10 (Miltenyi) and analysed with FlowJo10.

Fleury H., MacEachern M.K., Stiefel C.M., Anand R., Sempeck C., Nebenfuehr B., Maurer-Alcalá K., Ball K., Proctor B I.I.I., Belan O., Taylor E., Ortega R., Dodd B., Weatherly L., Dansoko D., Leung J.W., Boulton S.J, & Arnoult N. (2023). The APE2 nuclease is essential for DNA double strand break repair by microhomology-mediated end-joining. Molecular cell, 83(9), 1429-1445.e8.

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Profiling Islet-Infiltrating Leukocytes in NOD Mice

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To characterize islet infiltrating leukocytes and to determine their CD26 expression, pancreases from treated (n = 4) and control NOD mice (n = 4) were obtained 4 weeks after treatment. The experiment was replicated twice. Briefly, pancreases were perfused with collagenase (Collagenase CLS-4; Worthington Biochemical, Lakewood, NJ) through the common bile duct as described [25 (link)]. Pancreases were removed and incubated at 37°C for 15 min. After digestion, collagenase was neutralized with HBSS medium and the digested tissue was placed into a petri dish. Islets were hand-picked, mechanically homogenized and filtered with a 70 μm cell strainer to obtain a single cell suspension. For the identification of cell subsets, cell suspension was stained with a primary antibody Rat anti-mouse CD8 (clone Lyt-2.2; MyBiosource, San Diego, CA). After a single wash, a secondary antibody Goat anti-Rat FITC (SouthernBiotech, Birmingham, AL) was used. Finally, a third stage staining was performed with an anti-CD3 V450 (BD), anti-CD4 APC-Cy7 (BD) and anti-CD26 PE antibody (BioLegend, San Diego, California).

Alonso N., Julián M.T., Carrascal J., Colobran R., Pujol-Autonell I., Rodriguez-Fernández S., Teniente A., Fernández M.A., Miñarro A., Ruiz de Villa M.C., Vives-Pi M, & Puig-Domingo M. (2015). Type 1 Diabetes Prevention in NOD Mice by Targeting DPPIV/CD26 Is Associated with Changes in CD8+T Effector Memory Subset. PLoS ONE, 10(11), e0142186.

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