Measurements of tyrosine nitrosylation in protein of the hippocampus were carried out using the following:
1. ELISA from Hycult (#HK501)
2. ELISA from AFG Bioscience (#EK700342)
3. Confocal microscopy
The determination of NO in the hippocampus was carried out to test whether tyrosine nitrosylation in stressed mice is caused by increased NO.
Nitric oxide assay kit was from Abcam, Cambridge, UK (#ab272517)
Enzyme assays in the hippocampus were carried out to determine mechanisms of tyrosine nitrosylation:
1. Nitric-oxide-synthase activity: enzyme activity kit from Abcam (#ab211085)
2. Peroxidase activity assay: enzyme activity kit from Abcam (#ab155895)
3. Catalase activity assay: enzyme activity kit from Invitrogen (#EIACATC)
Behavioral studies were carried out to determine the functional role of tyrosine nitrosylation for MDD.
+ Open protocol