of human OR8H1 have been identified via NCBI (
NM_001005199.1), chimpanzee (NCBI reference sequence: XM_009440400.4),
and horse (NCBI reference sequence: NM_001391760.1) OR8H1 were amplified
from genomic DNA by polymerase chain reaction (PCR) using gene-specific
primers (
ligase (#M1804, Promega, Madison, USA) EcoRI/NotI
(#R6017/ #R6435, Promega, Madison, USA) into the expression plasmid
(#pFN210A SS-HaloTag CMV-neo Flexi-Vector, Promega, Madison, USA),
and verified by Sanger sequencing (Eurofins Genomics, Ebersberg, Germany)
using vector internal primers (
born to the Munich Zoological Garden before 2014. The horse DNA was
obtained from a saliva sample.
In the same way, all human OR
coding regions used in the present study (reference sequences and
selected genetic variants,
were purified using the PureYield Plasmid Midiprep System (#A2495,
Promega, Madison, USA).