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2 protocols using rabbit polyclonal anti human p65

1

Apoptosis Evaluation in Cell Cultures

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Cell culture medium (1640) and foetal bovine serum were purchased from Gibco (NY, USA). H4434 culture medium was purchased from stem cell. MTT, cremophor EL, and DMSO were purchased from Sigma Chemical Company (St. Louis, MA, USA). Cell lysis buffer was purchased from Beyotime Institute of Biotechnology (Beijing, China). AnnexinV-fluorescein isothiocyanate (FITC) and propidium iodide (PI) apoptosis detection kit, human CD34-APC, and human CD38-PE.cy7 antibody were purchased from BD (BD, USA). Rabbit polyclonal anti-human p65, XIAP, Bax, Bcl-2, JNK, p-JNK, ERK1/2, p-ERK1/2, p38, p-p38, c-Jun, p-c-Jun, c-Fos, c-Myc, PARP, caspase-3, caspase-9, and β-actin antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). ECL-Plus Kit was purchased from Thermo Scientific (Rockford, IL, USA). Kunming mice and NOD/SCID mice were purchased from Chinese Academy of Sciences (Shanghai, China).
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2

Apoptosis Pathway Analysis in Cells

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Cell culture medium reagents, MTT, and DMSO were purchased from Sigma Chemical Company (St. Louis, MA, USA). Rabbit polyclonal anti-human p65, XIAP, FLIP, Bax, Bcl-2, PARP, caspase-3, caspase-9, and β-actin antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Fetal bovine serum (FBS) was purchased from Gibco (NY, USA). Cell lysis buffer was purchased from Beyotime Institute of Biotechnology (Beijing, China). AnnexinV-FITC and PI apoptosis detection kit was purchased from BD (BD, USA). Human CD34-APC, CD38-PE.cy7, CD33-APC, CD19-PE, CD235a-FITC, and CD3-APC were purchased from BD (BD, USA). ECL-Plus Kit was purchased from Thermo Scientific (Rockford, USA).
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