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Stranded truseq mrna kits

Manufactured by Illumina
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The Illumina stranded TruSeq mRNA kits are a set of laboratory tools designed to prepare RNA samples for sequencing on Illumina platforms. The kits provide a standardized workflow for isolating and converting mRNA molecules into sequencing-ready libraries while preserving strand-specific information.

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Lab products found in correlation

Direct zol kit, by Zymo Research (2 mentions) Hiseq 2500, by Illumina (2 mentions) Novaseq 6000, by Illumina (2 mentions)

Close spelling variants of this product

TruSeq Stranded mRNA kit (698 citations) TruSeq Stranded mRNA (115 citations) TruSeq kits (44 citations) MRNA TruSeq kit (7 citations) TruSEQ Stranded kits (4 citations)

2 protocols using stranded truseq mrna kits

1

RNA-seq Library Preparation and Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was purified from Trizol with DIRECTzol kit (Zymo Research, Irvine, CA) according to manufacturer’s instructions. For data in Figures 3, 5A, 6B, 6G, S2, and S3, RNA-seq libraries were prepared using KAPA stranded mRNA-seq library kit per manufacturer’s instructions and single-end sequenced at read length 50 bp on an Illumina HiSeq 2500. For data in Figures 5D, 5E, 6C–F, RNA-seq libraries were generated by MedGenome, Inc. (Foster City, CA) using Illumina stranded TruSeq mRNA kits and paired-end sequenced at read length 150 bp on an Illumina NovaSeq 6000.
Kishimoto K., Wilder C.L., Buchanan J., Nguyen M., Okeke C., Hoffmann A, & Cheng Q.J. (2021). High Dose IFN-β Activates GAF to Enhance Expression of ISGF3 Target Genes in MLE12 Epithelial Cells. Frontiers in Immunology, 12, 651254.
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2

RNA Extraction and Sequencing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was purified from Trizol with DIRECTzol kit (Zymo Research, Irvine, CA) according to manufacturer's instructions. For data in Fig. 3b, Fig. 4g, Fig. 5, Fig. S2, and Fig. S3, RNA-seq libraries were prepared using KAPA stranded mRNA-seq library kit per manufacturer's instructions and single-end sequenced at read length 50 bp on an Illumina HiSeq 2500. For data in Fig. 3e-3d, Fig. 4a, and Fig. 4c-f, RNAseq libraries were generated by MedGenome, Inc. (Foster City, CA) using Illumina stranded TruSeq mRNA kits and paired-end sequenced at read length 150 bp on an Illumina NovaSeq 6000.
Kishimoto K., Wilder C.L., Buchanan J., Nguyen M.A., Okeke C., Hoffmann A., & Cheng Q.J. (2020). High dose IFN-βactivates GAF to enhance expression of ISGF3 target genes in epithelial cells.
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