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The EBRG1 is a compact bench-top refrigerated centrifuge designed for general laboratory use. It provides reliable and consistent performance for a variety of centrifugation applications.

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Lab products found in correlation

L2880, by Merck Group (1 mentions) Jes5 2a5, by Thermo Fisher Scientific (1 mentions) K18 hace2 2prlmn, by Jackson ImmunoResearch (1 mentions) A95 1, by BD (1 mentions) Golgiplug, by BD (1 mentions) Cytofix cytoperm, by BD (1 mentions) Monensin, by Thermo Fisher Scientific (1 mentions) Ly6g 1a8, by BioLegend (1 mentions) Ly6c hk1, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rat IgG1 k isotype control (clone eBRG1) (3 citations) Rat IgG1 κ isotype control (eBRG1) (2 citations) EBRG1 cat (2 citations)

4 protocols using ebrg1

1

IL-10 Modulation of C21 Effects in LPS-Induced Mice

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Mice were randomized into 7 groups (n = 6 each): i) Control (isotype), ii) C21, iii) LPS, iv) C21 + LPS, v) Neutralizing IL-10 antibody (IL-10Ab)+C21, vi) IL-10Ab + C21 + LPS vii) IL-10Ab + LPS. LPS (L2880, Sigma Aldrich) was given at a dose of 5 mg/kg. Rat IgG1 kappa isotype control (eBRG1, Thermo Fisher Scientific) and a neutralizing IL-10 monoclonal antibody (JES5-2A5, Thermo Fisher Scientific) were given a dose of 200 μg/mice in a total volume of 200 μL. IL-10 Ab treatment was given 1-h prior to C21, and LPS treatment was given 1-h after C21. Mice were euthanized 1-h post LPS.
Fatima N., Patel S, & Hussain T. (2021). Angiotensin AT2 Receptor is Anti-inflammatory and Reno-Protective in Lipopolysaccharide Mice Model: Role of IL-10. Frontiers in Pharmacology, 12, 600163.
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2

SARS-CoV-2 Infection in Transgenic hACE2 Mice

Cited 1 time
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8–16 week-old male Tg (K18-hACE2) 2Prlmn (Jackson Laboratories; ref. 20 (link)) mice were challenged with 5,000 PFU of SARS-CoV-2, Hong Kong/VM20001061/2020 (BEI Resources), in 50 μL by an i.n. route under 100 μL ketamine/xylazine sedation. Mice were followed daily for clinical symptoms, which included weight loss (scores 0–5), activity (scores 0–3), fur appearance and posture (scores 0–2), and eye closure (scores 0–2). Mice were given 150 μg of anti–IL-13 (Thermo Fisher Scientific, eBio1316H; catalog 16-7135-85) or an isotype matched control IgG (Thermo Fisher Scientific, eBRG1; catalog 16-4301-85) administered on day 0, 2, and 4 after infection. For experiments utilizing hyaluronidase, 14 U in 70 μL of ovine testicular hyaluronidase (Vitrase; 200 USP U/mL) or saline control were administered i.n. following isoflurane anesthetization on day 5 after infection. For anti-CD44 experiments, 100 μg of anti-CD44 (BD Biosciences, IM7; catalog 553131) or IgG2 (BD Biosciences, A95-1; catalog 559478) were administered on day 1 after infection, and then mice were given an additional 50 μg on days 2, 3, and 4.
Donlan A.N., Sutherland T.E., Marie C., Preissner S., Bradley B.T., Carpenter R.M., Sturek J.M., Ma J.Z., Moreau G.B., Donowitz J.R., Buck G.A., Serrano M.G., Burgess S.L., Abhyankar M.M., Mura C., Bourne P.E., Preissner R., Young M.K., Lyons G.R., Loomba J.J., Ratcliffe S.J., Poulter M.D., Mathers A.J., Day A.J., Mann B.J., Allen J.E, & Petri WA J.r. (2021). IL-13 is a driver of COVID-19 severity. JCI Insight, 6(15), e150107.
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3

Immune Response Analysis in Malaria Infection

Cited 1 time
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Blood was harvested 6 d post infection with P. berghei. Red blood cells were lysed with ACK and leukocytes were incubated with LPS (100 ng/μL) or vehicle plus GolgiPlug (BD) and monensin (eBioscience) for 4 h. Samples were blocked with anti-Fc receptor antibody (α-CD16/32, clone 2.4G2; UCSF Monoclonal Antibody Core) and labeled with antibodies to CD11b (M1/70) and F4/80 (BM8) (UCSF), Ly6c (HK1.4; eBioscience) and Ly6g (1A8; BioLegend). Following fixation and permeablization with Cytofix/Cytoperm (BD), intracellular labeling was performed with antibodies to IL-1β (NJTEN3), TNF (TN3-19), or rat IgG1 isotype (eBRG1) (all eBioscience). Samples were analyzed on an LSR II (BD). Classical monocytes were defined as CD11b+ Ly6g− F4/80int Ly6chi, as previously described (13 (link)).
Fontana M.F., Baccarella A., Kellar D., Oniskey T.K., Terinate P., Rosenberg S.D., Huang E.J., Herbert D.R, & Kim C.C. (2015). Myeloid expression of the AP-1 transcription factor JUNB modulates outcomes of type 1 and type 2 parasitic infections. Parasite immunology, 37(9), 470-478.
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4

Subcutaneous Delivery of TNF-α Antibodies

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For the delivery of TNFα neutralizing antibodies (MP6-XT3, 1 mg·mL−1, Invitrogen) or the IgG1 kappa isotype control (eBRG1, 1 mg·mL−1, eBioscience), a total of 200 μL of the undiluted TNFα antibody or IgG was loaded into each Alzet minipump. The pumps were implanted subcutaneously into 6-week-old male TLR9−/− or WT mice, and the treatment continued for a total of 6 weeks.
Ding P., Tan Q., Wei Z., Chen Q., Wang C., Qi L., Wen L., Zhang C, & Yao C. (2022). Toll-like receptor 9 deficiency induces osteoclastic bone loss via gut microbiota-associated systemic chronic inflammation. Bone Research, 10, 42.
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