About 60-100 µg of protein was loaded on each well of 10-15% SDS-polyacrylamide gel. The protein was transferred to a nitrocellulose membrane and blocked with casein buffer at room temperature for 1 hour. The following antibodies were added to the membrane and incubated at 4 °C overnight: rabbit polyclonal anti-ColI (abcam; cat no. ab34710), rabbit monoclonal anti-α-SMA (abcam; cat no. ab32575), rabbit polyclonal anti-TGFβ1 (abcam; cat no. ab92486), goat polyclonal anti-SCF (R&D; cat no.AF-455-NA), goat polyclonal anti-c-Kit (R&D; cat no.AF1356), rabbit monoclonal anti-Phospho-NF-κB p65 (CST company; cat no. 3033), and rabbit monoclonal anti-NF-κB p65 (CST company; cat no. 8242). HRPlabeled goat anti-rabbit or goat anti-mouse IgG (Santa Cruz Biotechnology) were used as secondary antibodies (dilution 1:1000). Target bands were detected by electrochemiluminescence (ECL). Bio-Rad Quantity One (29.0 version) software was used for semi-quantitative analysis.
Hrplabeled goat anti rabbit or goat anti mouse igg
Manufactured by Santa Cruz Biotechnology
HRP-labeled goat anti-rabbit or goat anti-mouse IgG is a secondary antibody conjugated with horseradish peroxidase (HRP). It is used to detect and quantify the presence of rabbit or mouse primary antibodies in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry.
Automatically generated - may contain errors
2 protocols using hrplabeled goat anti rabbit or goat anti mouse igg
1
Protein Analysis via Western Blotting
2
Protein Analysis via Western Blotting
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About 60-100 µg of protein was loaded on each well of 10-15% SDS-polyacrylamide gel. The protein was transferred to a nitrocellulose membrane and blocked with casein buffer at room temperature for 1 hour. The following antibodies were added to the membrane and incubated at 4 °C overnight: rabbit polyclonal anti-ColI (abcam; cat no. ab34710), rabbit monoclonal anti-α-SMA (abcam; cat no. ab32575), rabbit polyclonal anti-TGFβ1 (abcam; cat no. ab92486), goat polyclonal anti-SCF (R&D; cat no.AF-455-NA), goat polyclonal anti-c-Kit (R&D; cat no.AF1356), rabbit monoclonal anti-Phospho-NF-κB p65 (CST company; cat no. 3033), and rabbit monoclonal anti-NF-κB p65 (CST company; cat no. 8242). HRPlabeled goat anti-rabbit or goat anti-mouse IgG (Santa Cruz Biotechnology) were used as secondary antibodies (dilution 1:1000). Target bands were detected by electrochemiluminescence (ECL). Bio-Rad Quantity One (29.0 version) software was used for semi-quantitative analysis.
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