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Hplc grade water

Manufactured by Fujifilm
Sourced in Japan

HPLC-grade water is a high-purity water used in high-performance liquid chromatography (HPLC) applications. It is designed to meet the stringent requirements of HPLC analysis, ensuring consistent and reliable results.

Automatically generated - may contain errors

2 protocols using hplc grade water

1

Topotecan Quantification using LC-MS

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Topotecan was obtained from Sigma-Aldrich (St. Louis, MO, USA). Liquid chromatography–mass spectrometry (LC-MS)-grade acetonitrile was obtained from Merck (Darmstadt, Germany). Camptothecin, high-performance liquid chromatography (HPLC)-grade methanol, and HPLC-grade water were obtained from FUJIFILM Wako (Osaka, Japan). Dimethyl sulfoxide, acetic acid, ammonium acetate, sodium hydroxide (NaOH), and hydrochloric acid (HCl) were obtained from Nacalai Tesque (Kyoto, Japan).
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2

Illudin S Extraction and Analysis

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An illudin S solution (1000 μg/mL in methanol) was obtained from Hayashi Chemical Co., Inc (Osaka, Japan). HPLC-grade water, methanol, n-hexane, ethyl acetate, n-butanol and formic acid (FA, for HPLC 99%) were obtained from Wako Chemical Co., Inc. (Osaka, Japan). Purified water used for the mobile phase and sample preparation was obtained from a PURELAB Flex 5 system (ELGA, London, UK). A stock solution of Illudin S (100 μg/mL) was adjusted using methanol. The mushroom Omphalotus japonicus was sampled in 2017, and an extract was diluted by water/methanol (50/50, v/v) and stored at -20°C.
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