BrainSpheres were fixed with 2.5% glutaraldehyde in sodium cacodylate buffer, followed by sodium cacodylate buffer washing steps. Samples were post-fixed with 1% osmium tetroxide, 5 mM calcium chloride and 1.25% potassium ferrocyanide in sodium cacodylate buffer, followed by cacodylate buffer and distilled water washing steps. After gradual dehydratation with acetone and embedding in Epoxy resin (PolyBed812, Polysciences), ultrathin sections were obtained with Leica C7 ultramicrotome, collected with copper grids and stained with uranyl acetate and lead citrate. Grids were observed with 120 kV in FEI Tecnai Spirit TEM.
C7 ultramicrotome
Manufactured by Leica
The C7 ultramicrotome is a precision instrument used for the preparation of ultra-thin sections for transmission electron microscopy (TEM) analysis. It is designed to cut samples into extremely thin slices, typically in the range of 50-100 nanometers (nm) in thickness, allowing for high-resolution imaging and analysis of cellular structures and materials at the nanoscale level.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using c7 ultramicrotome
1
Ultrastructural analysis of BrainSpheres
2
Ultrastructural Analysis of BrainSpheres
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BrainSpheres were fixed with 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4)
for 2 h at RT. After three washing steps with sodium cacodylate buffer (pH 7.4), the samples were post-fixed with 1% osmium tetroxide, 5 mM calcium chloride and 1.25% potassium ferrocyanide in 0.1 M sodium cacodylate buffer for 1 h in the dark, followed by three washing steps with 0.1 M cacodylate buffer and distilled water. The BrainSpheres were gradually dehydrated with acetone and embedded in Epoxy resin (PolyBed812, Polysciences). Ultrathin sections from the surface or core of the BrainSpheres were obtained with a Leica C7 ultramicrotome, collected with copper grids and stained with uranyl acetate and lead citrate.
Grids were then observed in a 120 kV in a FEI Tecnai Spirit TEM.
for 2 h at RT. After three washing steps with sodium cacodylate buffer (pH 7.4), the samples were post-fixed with 1% osmium tetroxide, 5 mM calcium chloride and 1.25% potassium ferrocyanide in 0.1 M sodium cacodylate buffer for 1 h in the dark, followed by three washing steps with 0.1 M cacodylate buffer and distilled water. The BrainSpheres were gradually dehydrated with acetone and embedded in Epoxy resin (PolyBed812, Polysciences). Ultrathin sections from the surface or core of the BrainSpheres were obtained with a Leica C7 ultramicrotome, collected with copper grids and stained with uranyl acetate and lead citrate.
Grids were then observed in a 120 kV in a FEI Tecnai Spirit TEM.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!