Cells were harvested and lyzed using NP-40-based lysis buffer with protease and phosphatase inhibitors. Total protein (500 μg) in 1 ml lysate was incubated with 2 μg of anti-NHE1 antibody (Abcam, UK) or anti-FLT3 antibody (Santa Cruz Biotechnology, TX, USA) [45 (link)] at 4 °C overnight. The antibody-protein complex was pulled down using Protein G sepharose (ThermoFisher Scientific) at 4 °C for 2 hours. The sepharose was washed twice with lysis buffer and eluted by SDS protein loading dye by boiling for 5 min. The supernatant was collected and stored at −80 °C for further analysis.
Anti flt3 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom, United States
The Anti-FLT3 antibody is a laboratory reagent designed for research purposes. It is a monoclonal antibody that specifically binds to the FLT3 (Fms-like tyrosine kinase 3) protein. FLT3 is a receptor tyrosine kinase that plays a role in the regulation of cell growth and differentiation. This antibody can be used in various applications, such as Western blotting, immunoprecipitation, and flow cytometry, to detect and study the FLT3 protein.
Automatically generated - may contain errors
2 protocols using anti flt3 antibody
1
Immunoprecipitation of Cellular Proteins
2
Immunofluorescence Staining of FLT3
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were grown on coverslips and following methanol fixation, blocking was performed at room temperature for 1 h. Anti-FLT3 antibody (Santa Cruz; 1:200) incubation was done at room temperature for 1 h. Alexa Fluor 568-tagged anti-rabbit IgG secondary antibody (Invitrogen, USA) (1:1000) was used for another 1 h at room temperature. Coverslips were mounted with UltraCruz mounting medium with DAPI (Santa Cruz) for counter staining. Slides were observed with a fluorescent microscope (Leica TCS/SP5). The excitation wavelengths for Alexa Fluor 568 and DAPI were 578 nm and 359 nm, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!