Soybean lecithin

1,2-Distearoyl-sn-glycero-3-phosphoethanol-amine–N-[methoxy (polyethylene glycol)-3000] ammonium salt (mPEG3000–DSPE), 1,2-distearoyl-sn-glycero-3-phos-phoethanolamine-N-[methoxy (polyethylene glycol)-350] ammonium salt (mPEG350–DSPE), and DTPA–dis-(stearyleamide) (gadolinium salt) (Gd–DTPA–DSA) were from Avanti Polar Lipids Inc. Soybean lecithin was purchased from MP Biomedicals, LLC. 1,2-Distearoyl-sn-glycero-3-phos-phoethanolamine–N-[methoxy (polyethylene glycol)-1000] biotin (biotin–PEG1000–DSPE) was purchased from Creative PEGWorks. MMP2-cleavable lipid (mPEG2000-MMP2p-DSPE) and c[RGDfK]-PEG18-DSPE were synthesized by SyMO-Chem (Eindhoven, The Netherlands, www.symo-chem.nl) as previously reported.²⁹ 4-Amino-phenyl-mercuric acetate (APMA) and Poly(D,L-lactide-co-glycolide) (PLGA) with a 50:50 monomer ratio and a 30 000–60 000 Da molecular weight were acquired from Sigma-Aldrich. PLGA conjugation with Cy5.5, Cy5, or Cy3.5 was obtained as previously reported.^{40 (link)} Dulbecco’s modified Eagle medium (DMEM) was from Gibco and Invitrogen. Human umbilical vein endothelial cells and bullet kit endothelial basal medium (EBM-2) were purchased from Lonza (Allendale, NJ). Human MMP2 (hMMP2) was purchased from R&D Systems. MDA-MB-231 expressing green fluorescent protein (GFP) was a gift from Dr. Aaronson (Icahn School of Medicine, New York, NY).

1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-carboxy (polyethylene glycol) 2000 (DSPE–PEG2000–COOH) and the cross-linkable lipid (1-palmitoyl-2-(10,12-tricosadiynoyl)-sn-glycero-3-phosphocholine) (PTPC) were obtained from Avanti Polar Lipids (Alabaster, AL). Docetaxel (Dtxl), wortmannin (Wtmn), etoposide and gemcitabine hydrochloride were purchased from Sigma-Aldrich (St. Louis, MO). Olaparib was purchased from Selleckchem (Houston, TX). PLGA (poly(D,L-lactide-co-glycolide)) with a 50 : 50 monomer ratio, ester terminated, and viscosity of 0.72–0.92 dl g⁻¹ was purchased from Durect Corporation (Pelham, AL). Soybean lecithin consisting of 90–95% phosphatidylcholine was obtained from MP Biomedicals (Solon, OH). 200 Proof Ethanol (Molecular Biology Grade) and Acetonitrile (HPLC Grade) were purchased from Fisher Scientific (Hampton, NH). Dulbeco’s Phosphate Buffer Saline (PBS, 1X) was purchased from Gibco by Life Technologies (Carlsbad, CA). H460, H23 and H69AR cell lines were purchased from UNC’s Lineberger tissue culture facility.