Frozen secions were immersed in PBS, while paraffin sections were dewaxed, rehydrated, then immersed in PBS. Hematoxylin and eosin (H&E) staining was performed using Meyer’s hematoxylin solution (Muto Pure Chemicals, Tokyo, Japan) and 1% eosin in water (Fujifilm-Wako, Osaka, Japan). For Alcian blue staining, sections were rehydrated, stained with 1% Alcian blue 8GX (Sigma-Aldrich-Japan, Tokyo, Japan) in 3% acetic acid for 15 min, and stained with Meyer’s hematoxylin solution for 1 min, and 0.1% eosin in DW for 1 min. Microscopic images were obtained using a Nikon AZ-100 (Tokyo, Japan) and a Sony a7RII (Tokyo, Japan).
Meyer s hematoxylin solution
Manufactured by Muto Pure Chemicals
Sourced in Japan
Meyer's hematoxylin solution is a staining reagent used in histological and cytological laboratory procedures. It is a nuclear stain that selectively binds to the DNA and RNA in cell nuclei, producing a blue-purple color. The solution contains hematoxylin, an organic compound derived from the heartwood of the logwood tree, which is the primary staining agent.
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Lab products found in correlation
A7rii, by Sony (1 mentions)
Alcian blue, by Merck Group (1 mentions)
Meyer s hematoxylin solution, by Merck Group (1 mentions)
Alcian blue 8gx, by Merck Group (1 mentions)
Mildform 10n, by Fujifilm (1 mentions)
F4 80, by Cedarlane (1 mentions)
Oil red o staining, by Nacalai Tesque (1 mentions)
Bz x viewer, by Keyence (1 mentions)
Bz x analyzer, by Keyence (1 mentions)
Bz x710, by Keyence (1 mentions)
2 protocols using meyer s hematoxylin solution
1
Histological Analysis of Tissue Sections
2
Histological Analysis of Macrophage Accumulation
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Samples were fixed for 20 h in Mildform 10N (Wako), embedded in paraffin, and then subjected to hematoxylin and eosin staining (Meyer’s hematoxylin solution and 1% eosin, Muto Pure Chemicals Co., Ltd., Tokyo, Japan) as well as immunohistochemistry using primary antibodies against F4/80 (Cedarlane Laboratories, Ontario, Canada). Oil Red O staining (Nacalai Tesque) was performed on frozen sections. For the image analysis, a microscope BZ-X710 (Keyence, Osaka, Japan), BZ-X Viewer version 1.3.1.1 (Keyence), and BZ-X Analyzer version 1.3.1.1 (Keyence) were used.
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