Bioscope 1 microscope

AFM images were acquired with a BioScope I microscope equipped with a Nanoscope IIIA controller (Veeco Metrology, Plainview, NY, USA). The BioScope head was mounted on the top of the samples which have been previously physically attached to the underlying substrate to avoid eventual vibrations potentially harmful for the tip. AFM images were acquired in tapping mode in air, at room temperature, by using triangular doped silicon cantilevers (Veeco, NTESP) with nominal spring constants between 20 and 80 N/m and a resonance frequency around 270 KHz. Processing of AFM images as well as quantification of the roughness were carried out using the free software Gwyddion (version 2.41).

A BioScope I microscope equipped with a Nanoscope IIIA controller (Veeco Metrology, Plainview, NY, USA) was used to acquire AFM topography images. In order to reduce vibrations or movements which negatively affect the tip scan, all the samples were sticked on the underlying substrate.
The BioScope head was then mounted on the top of the samples. AFM imaging was performed in non-contact mode at room temperature, in air; triangular doped silicon cantilevers (Veeco, NTESP) with nominal spring constants between 20 and 80 N/m and a resonance frequency around 270 KHz were used. AFM images post-processing and root mean square (RMS) roughness quantification were obtained by using the free software Gwyddion (v. 2.41).