Biowhittaker alpha minimum essential medium

BM- and A-hMSCs were isolated and characterized following high-quality standards as described previously [31 (link),32 (link)]. Briefly, the characterization of BM-hMSCs comprised analysis of expression (CD105, CD73, and CD90) or absence (CD45, CD34, CD3, and HLA-DQ, -DP, -DR) of specific surface markers, plastic-adherent growth, and trilineage differentiation following the ISCT minimal criteria [33 (link)]. A-hMSCs were analyzed for expression (CD13, CD105, CD73, CD90) and absence (CD45, CD34, CD14, HLA-DQ, -DP, -DR) of specific surface markers based on the IFATS and the ISCT joint statement [33 (link)]. Human MSCs were propagated in BioWhittaker Alpha Minimum Essential Medium (Lonza, Basel, Switzerland, #BE02-002F) supplemented with 5% (A-hMSCs) or 8% (BM-hMSCs) irradiated human platelet lysate and 500 units of heparin (Ratiopharm, Ulm, Germany) [34 (link)]. Human MSCs were cultivated adherently on cell culture plates (ThermoFisher Scientific, #168381) at 37 °C, 90% relative humidity, and 5% CO₂. TrypLE select (ThermoFisher Scientific, #12563029) was used to detach hMSCs from the plates. Human MSCs were used for experiments up to passage 8. All experiments were performed with hMSCs isolated from three healthy donors.