Following antibodies were used in this study: Anti-human MELK antibody (in-house, previously described [14 (link)]), anti-human DEPDC1 (in-house, previously described [36 (link)]), anti-human FOXM1, anti-human p53 (Santa Cruz Biotechnology), anti-human Ki67 (Millipore), anti-human p21, anti-human Slug, anti-human Snail (Cell Signaling Technology), anti-human E-cadherin (BD biosciences), anti-HA (Roche), anti-FLAG M2, and anti-beta-actin antibodies (Sigma). OTS167 was dissolved in DMSO (Sigma) for cell culture or in 5% glucose solution for animal study. The target sequences of oligo siRNAs were 5′-CUUACGCUGAGUACUUCGAUU-3′ for MELK and 5′-AGUUCAUUGGAACUACCAAUU-3′ for Luciferase (control). DEPDC1 oligo siRNA was purchased from Santa Cruz Biotechnology (sc-78918).
Anti human e cadherin
Manufactured by BD
Anti-human E-cadherin is a laboratory equipment product used for the detection and analysis of E-cadherin, a cell-cell adhesion protein, in human samples. It is a specific antibody that binds to E-cadherin, allowing for its identification and quantification.
Automatically generated - may contain errors
Lab products found in correlation
Anti human foxm1, by Santa Cruz Biotechnology (1 mentions)
Anti human p53, by Santa Cruz Biotechnology (1 mentions)
Anti human slug, by Cell Signaling Technology (1 mentions)
Anti human snail, by Cell Signaling Technology (1 mentions)
Anti β actin antibody, by Merck Group (1 mentions)
Anti ha, by Roche (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
Ab12169, by Abcam (1 mentions)
Ab205719, by Abcam (1 mentions)
Ab18251, by Abcam (1 mentions)
Anti human nf κb p65 d14e12, by Cell Signaling Technology (1 mentions)
2 protocols using anti human e cadherin
1
Antibody and Inhibitor Usage in MELK Study
2
Comprehensive Antibody Panel for Cell Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Antibodies used were anti-human-fibronectin (Invitrogen, MA5-11981), anti-human E-cadherin (BD Biosciences, 610182), anti-human N-cadherin (D4R1H) (Cell Signaling, 13116S), anti-human phospho-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (D27F4) (Cell Signaling, 8828S), anti-human phospho-SMAD2 (Ser465/467) (Cell Signaling, 3108S), anti-human SMAD2/SMAD3 (D7G7) (Cell Signaling, 12470S), anti-human phospho-FAK (Tyr397) (D20B1) (Cell Signaling, 8556), anti-human FAK (Cell Signaling, 3285), anti-human TNIK (Cell Signaling, 32712), anti-human NF-κB p65 (D14E12) (Cell Signaling, 8242S), anti-human phospho-NF-κB p65 (Ser536) (93H1) (Cell Signaling, 3033S), anti-human β-catenin (Cell Signaling, 9562), anti-human α-tubulin (Abcam, ab18251), anti-human HDAC2 (Abcam, ab12169), anti-human histone 3 (Abcam, ab176842), anti-α-SMA antibodies (Cell Signaling, 19245; Abcam, ab5694; Abcam, ab32575), anti-GAPDH (Absin, 016D), anti-collagen I antibody (Abcam, ab34710; LSL), goat anti-mouse IgG (Abcam, ab205719), goat anti-rabbit IgG (Abcam, ab205718) and goat polyclonal antibodies conjugated to HRP (DAKO, K4003; Vector Lab, PI-1000).
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