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Potassium acetate

Manufactured by Thermo Fisher Scientific
Sourced in Belgium, China

Potassium acetate is a chemical compound that can be used as a laboratory reagent. It is a white, crystalline solid that is soluble in water and alcohol. Potassium acetate has various applications in scientific and industrial settings.

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21 protocols using potassium acetate

1

Phytochemical and Antioxidant Assays

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All the chemicals used in this study were of laboratory grade. The Folin–Ciocalteu's phenol reagent (FCR), α-amylase and acarbose were obtained from Sigma Aldrich, St. Louis, MO, USA. The solvent ethanol and methanol (Qualigens Fine Chemicals) were purchased from the local city of Kathmandu, Nepal. Sulphuric acid, hydrochloric acid, aluminium chloride, sodium chloride, sodium carbonate, dimethyl sulphoxide, potassium acetate, disodium hydrogen phosphate dihydrate, and sodium dihydrogen phosphate dihydrate (Thermo-Fisher Scientific India) were purchased from Kathmandu, Nepal. quercetin and gallic acid (Hi-media Laboratories) were procured from the local city of Kathmandu, Nepal. The 1,1-diphenyl-2-picrylhydrazyl (DPPH), gallic acid and quercetin (Wako Pure Chemicals, Osaka, Japan), were purchased from the local city of Kathmandu, Nepal.
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2

Colloidal Rare-Earth Nanocrystal Synthesis

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Cesium acetate (CsOAc, 99.9%), 1-octadecene (ODE, 90%), oleic acid (OA, 90%), oleylamine (OAm, 70%), lead chloride (PbCl2, 99.99%), europium acetate hydrate (Eu(OAc)3.3H2O, 99.99%), cerium acetate hydrate (Ce(OAc)3.4H2O, 99.99%), erbium acetate hydrate (Er(OAc)3.4H2O, 99.99%), samarium acetate hydrate (Sm(OAc)3.6H2O, 99.99%), potassium acetate (KOAc, 99.9%, ACS grade, Fischer), ethanol (99%), zinc acetate (99.99%), sodium hydroxide (NaOH, 99.99%), toluene (99,95%), were all purchased from Sigma-Aldrich and were used as starting materials without further purification.
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3

RecA Protein Purification and Characterization

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RecA protein from E. coli at a concentration of 2 mg/mL was obtained from New England Biolabs Inc. (Ipswich, USA). SYBR Gold Nucleic Acid Gel Stain, XapI enzyme (recognition sequence 5′…R^AATTY…3′, 10 units/μL) and 1X tango buffer containing tris acetate (TAc) (33 mM, pH 7.9), magnesium acetate (MgAc) (10 mM) potassium acetate (66 mM), and BSA (0.1 mg/mL) were purchased from Thermo Fisher Scientific Inc. (Waltham, USA). Proteinase K (>800 units/mL), ammonium persulphate, TAc, MgAc (1 M), tris-EDTA (TE) buffer (100X), N,N,N′,N′-tetramethylethylenediamine (TEMED) and acrylamide/bis-acrylamide (30%) were obtained from Sigma-Aldrich (St. Louis, USA). Synthetic oligomers were purchased from Integrated DNA Technologies (Leuven, Belgium) and were stored at a concentration of 100 μM in 1x TE buffer at −20 °C. Blue/Orange loading dye (6X) was obtained from Promega Corporation (Madison, USA).
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4

Comprehensive Reagents and Procedures

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Folin–Ciocalteau’s reagent, bovine serum albumin, ABTS, acarbose, sodium dodecyl sulfate, EDTA, sodium bicarbonate, α-glucosidase, iNOS, TNF-α, IL-6, acetylcholinesterase, lipopolysaccharide, acetylcholine iodide, DTNB, TPTZ, chlorogenic acid, DMSO, and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glycine was purchased from J.T.Baker (Phillipsburg, NJ, USA). DPPH, phenazine methosulphate, and nitroblue tetrazolium were supplied from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Thiazolyl blue tetrazolium bromide was purchased from MedChemExpress (Monmouth Junction, NJ, USA). Butyl hydroxytoluene, nicotinamide adenine dinucleotide, sodium acetate, and potassium acetate were supplied from Acros Organics (Geel, Belgium). Ammonium persulfate and TEMED were purchased from Bio-Rad (Hercules, CA, USA). Tween20 was purchased from Merck (Darmstadt, Germany). Acetic acid was supplied from Macron Fine Chemicals (Center Valley, PA, USA). Iron (III) chloride, aluminum chloride, as well as p-Nitro-phenyl-α-D-glucopyranoside were purchased from Alfa Aesar (Lancashire, UK). Disodium hydrogenphosphate, sodium carbonate, potassium peroxodisulfate, and potassium dihydrogenphosphate were obtained from SHOWA Chemical Co. (Sun Valley, CA, USA).
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5

Quercetin Bioactivity Evaluation Protocol

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Quercetin was purchased from MedChemExpress (Monmouth Junction, NJ, USA). 2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), Folin–Ciocalteu reagent, tyrosinase, gallic acid, α-glucosidase, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), bovine serum albumin, and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). Disodium hydrogen phosphate, potassium peroxodisulfate, sodium carbonate, and sodium dihydrogen phosphate were purchased from SHOWA Chemical Co. Ltd. (Chuo-ku, Japan). Ferric chloride (FeCl3), p-nitro-phenyl-α-D-glucopyranoside (p-NPG), and aluminum chloride (AlCl3) were obtained from Alfa Aesar (Lancashire, UK). 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), nitroblue tetrazolium (NBT), and phenazine methosulphate (PMS) were supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Potassium acetate, acarbose, sodium acetate, nicotinamide adenine dinucleotide (NADH), and butyl hydroxytoluene (BHT) were acquired from Acros Organics (Geel, Belgium). Arbutin was purchased from AK Scientific. Acetic acid was supplied by Avantor Performance Materials, LLC. (Radnor, PA, USA).
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6

Antioxidant and Enzyme Inhibition Assays

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Gallic acid, Folin–Ciocalteu reagent, hydrogen peroxide solution, α-glucosidase, acetylthiocholine iodide, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), acetylcholinesterase, 5,5′-dithio-bis(2-nitrobenzoic) acid, 2,4,6-tripyridyl-s-triazine, and Trolox were bought from Sigma-Aldrich. Nitroblue tetrazolium, 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl, loroglucinol, and phenazine methosulphate were supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Aluminum chloride, ferric chloride, and p-nitrophenyl-α-D-glucopyranoside were bought from Alfa Aesar. Potassium dihydrogenphosphate, sodium carbonate, potassium peroxodisulfate, disodium hydrogenphosphate, and sodium carbonate were bought from the Showa Chemical Industry Co., Ltd. (Tokyo, Japan). Sodium acetate, butyl hydroxytoluene, potassium acetate, and nicotinamide adenine dinucleotide were supplied by Acros Organics. Galanthamine hydrobromide was bought from MedchemExpress.
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7

Antioxidant Capacity Evaluation Protocol

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Ascorbic acid, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), α-glucosidase, ethylenediaminetetraacetic acid (EDTA), gallic acid, Folin-ciocalteau’s reagent, hydrogen peroxide solution, Trolox, and 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ferric chloride (FeCl3) and p-nitro-phenyl-α-D-glucopyranoside (p-NPG) were obtained from Alfa Aesar (Lancashire, UK). Disodium hydrogenphosphate, potassium peroxodisulfate, sodium carbonate, and potassium dihydrogenphosphate were acquired from the SHOWA Chemical Co. Ltd. (Chuo-ku, Japan). 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH), deoxyribose, nitroblue tetrazolium (NBT), phenazine methosulphate (PMS), and 2-thiobarbituric acid (TBA) were supplied by Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Butyl hydroxytoluene (BHT), acarbose, nicotinamide adenine dinucleotide (NADH), sodium acetate, trichloroacetic acid (TCA), and potassium acetate were acquired from Acros Organics (Geel, Belgium). Acetic acid was supplied by Avantor Performance Materials, LLC (Radnor, PA, USA). Sodium hydroxide solution was acquired from Merck (Darmstadt, Germany).
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8

Antioxidant and Enzyme Inhibition Assays

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Folin–Ciocalteau reagent, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ascorbic acid, ethylenediaminetetraacetic acid (EDTA), α-glucosidase, acetylcholinesterase (AChE), acetylcholine iodide (AchI), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). Galanthamine and quercetin were purchased from MedChemExpress (Monmouth Junction, NJ, USA). p-Nitro-phenyl-α-D-glucopyranoside (p-NPG), aluminum chloride (AlCl3), and iron (III) chloride were purchased from Alfa Aesar (Lancashire, UK). Sodium carbonate, potassium peroxodisulfate, potassium dihydrogenphosphate, and disodium hydrogenphosphate were supplied by SHOWA Chemical Co., Ltd. (Chuo-ku, Japan). Loroglucinol, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH), phenazine methosulphate (PMS), nitroblue tetrazolium (NBT), and deoxyribose were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Butyl hydroxytoluene (BHT), nicotinamide adenine dinucleotide (NADH), sodium acetate, and potassium acetate were obtained from Acros Organics (Geel, Belgium). Acetic acid was purchased from Macron Fine Chemicals (Center Valley, PA, USA).
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9

Phytochemical Analysis of Plant Extracts

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HPLC 99% pure methanol obtained from Honeywell (Paris, France) was used as an extraction solvent, while the Folin–Ciocalteu reagent obtained from Fisher Scientific UK (Loughborough, UK) was used for spectrophotometric determination of total phenols. Hydrochloric acid (37%, w/w), sulfuric acid (96%, p.a.), sodium carbonate, anhydrous (99.5–100.5%), and formic acid (98%, p.a.) were obtained from Lach-ner (Neratovice, Czech Republic). Ethanol (96% pure) was obtained from Gram-mol (Zagreb, Croatia). Quercetin (95%), potassium acetate (99%), and aluminum chloride (98.5%) were purchased from Acros Organics (Guangzhou, China). Gallic acid standard (97.5–102.5%), DPPH (2,2-diphenyl-1-picrylhydrazyl radical), and TPTZ (2,4,6-tris-2-pyridyl-s-triazine) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Vanillin (99%), potassium chloride (99.0–100.5%), sodium acetate anhydride (99%), and chlorogenic acid (min. 95%) were purchased from Thermo Fisher (Kandel, Germany). Iron (III)-chloride hexahydrate and sodium acetate trihydrate resistant to potassium permanganate were obtained from Kemika (Zagreb, Croatia). Glacial acetic acid (≥99.8%) was purchased from Honeywell Fluka TM (Seelze, Germany) and trolox standard (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) used for the DPPH and FRAP method was purchased from Biosynth (Bratislava, Slovakia).
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10

Comprehensive Analysis of Phytochemicals

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HPLC 99% pure methanol was obtained from Honeywell (Paris, France). Folin-Ciocalteau (FC) reagent was purchased from Fisher Scientific UK (Loughborough, UK). Sodium carbonate, anhydrous (99.5–100.5%), hydrochloric acid (37%, w/w), sulfuric acid (96%, p.a.), and formic acid (98%, p.a.) were purchased from Lach-Ner (Neratovice, Czech Republic). Chlorogenic acid (min. 95%) and vanillin (99%) were purchased from Thermo Fisher (Kandel, Germany). Sodium acetate trihydrate resistant to potassium permanganate and iron (III)-chloride hexahydrate were purchased from Kemika (Zagreb, Croatia). Ethanol (96% pure) was obtained from Gram-mol (Zagreb, Croatia). Potassium acetate (99%), quercetin (95%), and aluminum chloride (98.5%) were obtained from Acros Organics (Guangzhou, China). DPPH (2,2-diphenyl-1-picrylhydrazyl radical), TPTZ (2,4,6-tris-2-pyridyl-s-triazine), and gallic acid standard (97.5–102.5%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glacial acetic acid (≥99.8%) was obtained from Honeywell FlukaTM (Seelze, Germany), and Trolox standard (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) used for DPPH and FRAP method was obtained from Biosynth (Bratislava, Slovakia).
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