Hrp labeled goat anti mouse antibody

Manufactured by Thermo Fisher Scientific

The HRP-labeled goat anti-mouse antibody is a secondary antibody used in immunoassays and other immunochemical techniques. It is generated by conjugating horseradish peroxidase (HRP) to a goat-derived antibody that specifically recognizes and binds to mouse primary antibodies. This antibody can be used to detect and quantify the presence of mouse-derived proteins or other analytes in a sample.

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Lab products found in correlation

Hrp labeled goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Ab2770, by Abcam (1 mentions) Supersignal west femto sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Hrp labeled donkey anti goat antibody, by Santa Cruz Biotechnology (1 mentions) Anti p18, by Thermo Fisher Scientific (1 mentions) Anti involucrin, by Merck Group (1 mentions) Anti blimp1, by Cell Signaling Technology (1 mentions) Anti bmrf1, by Merck Group (1 mentions) Anti c myc, by Abcam (1 mentions) Anti klf4, by Cell Signaling Technology (1 mentions) Anti bzlf1, by Santa Cruz Biotechnology (1 mentions) Anti δnp63, by BioLegend (1 mentions)

Close spelling variants of this product

Goat anti-mouse HRP (78 citations) HRP-anti-mouse (6 citations) HRP-labeled goat anti-mouse IgG or IgG2c Fc antibody (3 citations) Horseradish peroxidase (HRP)-labeled goat anti-mouse secondary antibody (2 citations)

3 protocols using hrp labeled goat anti mouse antibody

Immunohistochemical Analysis of STC2 Expression

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Immunohistochemistry (IHC) was used to assess the expression of STC2 according to the instruction of IHC protocol. The specimens were probed with anti-STC2 (1:200). Antibodies against STC2 (Cat# 60063-1-Ig) and PCNA (Cat# 10205-2-AP) were acquired from Proteintech technology. HRP-labeled goat anti-rabbit antibody and HRP-labeled goat anti-mouse antibody were purchased from Thermo Fisher Scientific.

Wu Z., Cheng H., Liu J., Zhang S., Zhang M., Liu F., Li Y., Huang Q., Jiang Y., Chen S., Lv L., Li D, & Zeng J.Z. (2022). The Oncogenic and Diagnostic Potential of Stanniocalcin 2 in Hepatocellular Carcinoma. Journal of Hepatocellular Carcinoma, 9, 141-155.

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Immunoblot Analysis of HIF-1α and AhR

Cited 1 time

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Immunoblot analysis was carried out as previously described [21 (link)] using 3-5×10⁵ cells. Rabbit anti-human HIF-1α (cat. # 3716S, Cell Signaling, Danvers, MA) and mouse anti-human AhR (cat. # ab2770, Abcam, Cambridge, MA) or aryl hydrocarbon receptor nuclear translocator (ARNT, clone # 775146, R&D Systems) primary antibodies were applied at 1/2,000, 1/5,000 and 2 μg/ml respectively. HRP-labeled goat anti-mouse (for AhR and ARNT, Thermo Fisher Scientific) and mouse anti-rabbit (for HIF-1α, Thermo Fisher Scientific) secondary antibodies (Thermo Fisher Scientific) were used at 1/50,000. Bands were visualized using Super Signal West Femto Sensitivity Substrate (Thermo Fisher Scientific) according to the manufacturer’s instructions. For immunoblot normalization, the same cell lysates were also tested for β-actin (cat. # ab8226, Abcam) antibodies at 1/10,000 and, subsequently, with an HRP-labeled goat anti-mouse antibody at 1/20,000 (Thermo Fisher Scientific). Band density was measured by Image J software.

Xie A., Robles R.J., Mukherjee S., Zhang H., Feldbrügge L., Csizmadia E., Wu Y., Enjyoji K., Moss A.C., Otterbein L.E., Quintana F.J., Robson S.C, & Longhi M.S. (2018). HIF-1α-induced xenobiotic transporters promote Th17 responses in Crohn’s disease. Journal of autoimmunity, 94, 122-133.

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Detailed Antibody Use for Immunoblot Analysis

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The following antibodies were used for immunoblot analyses in this study: anti-R rabbit polyclonal antibody directed against the R peptide (peptide sequence EDPDEETSSQAVKALREMAD 1: 2500), anti-p63ɑ (Cell Signaling Technologies catalog # 13109 1:1000), anti-ΔNp63 (Biolegend, catalog #619001), anti-KLF4 (Cell Signaling Technologies, catalog #4038 1: 1000), anti-BZLF1 (Santa Cruz, catalog # sc-53904, 1:500), anti-BMRF1 (Millipore, catalog # MAB8186, 1: 2500), anti-p18 (Thermo-Fisher Scientific, # PA1-73003, 1:2000), anti-BLIMP1 (Cell Signaling Technologies catalog # 9115S 1:1000), anti-involucrin (Sigma, catalog #19018, 1:3000), anti-c-myc (Abcam catalog# ab32072, 1:10000), p38 MAPK14 antibody (Cell Signaling Technologies catalog#9218 1:1000) and phospho-p38 MAPK (Cell Signaling Technologies catalog #9211 1:1000), anti-EBV SM polyclonal rabbit antibody (1:750), a generous gift from Sankar Swaminathan of the University of Utah, anti-Tristetraprolin (Cell Signaling Technologies catalog# #71632 1:1000). The secondary antibodies used were Horseradish peroxide (HRP)- labeled goat anti-mouse antibody (Thermo Scientific# 31430, 1:5000), HRP- labeled donkey anti-goat antibody (Santa Cruz#sc-2056, 1:5000), and HRP- labeled goat anti-rabbit antibody (Fisher Scientific 1:10000).

Van Sciver N., Ohashi M., Nawandar D.M., Pauly N.P., Lee D., Makielski K.R., Bristol J.A., Tsao S.W., Lambert P.F., Johannsen E.C, & Kenney S.C. (2021). ΔNp63α promotes Epstein-Barr virus latency in undifferentiated epithelial cells. PLoS Pathogens, 17(11), e1010045.

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