Wm3211

Four human melanoma cell lines carrying distinct genomic mutations (A375: BRAF^V600E/PTEN^WT/CKIT^WT; Sk-mel-28: BRAF^V600E/PTEN^T167A/CKIT^WT; wm115: BRAF^V600E/PTEN^WT/CKIT^WT; and wm3211: BRAF^WT/PTEN^WT/CKIT^L576P) were used for the study [23 (link),24 ]. A375, wm115, Sk-mel-28, and HEK-293 were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA), and wm3211 was obtained from Rockland Immunochemicals (Limerick, PA, USA). Cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, #11995073; Gibco, Waltham, MA, USA) (A375) or Eagle’s Minimum Essential Medium (EMEM) (wm115, Sk-mel-28, HEK-293) with 10% fetal bovine serum (FBS, #26140079; Gibco, Waltham, MA, USA), or Tumor Specialized Media with 2% FBS (wm3211). Cell culture media were supplemented with 10% FBS (Corning, Corning, NY, USA). Cells were cultured in a humidified atmosphere in a 5% CO₂ incubator maintained at 37 °C.

The human melanoma cell lines A375, wm115, and Sk-Mel-28 were obtained from American Type Culture Collection (Manassas, VA), and wm3211 was obtained from Rockland Immunochemicals (Limerick, PA). Cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; #11995073; Gibco, Waltham, MA) (A375) or Eagle’s Minimum Essential Medium (EMEM) (wm115, Sk-Mel-28) with 10% fetal bovine serum (FBS; #26140079; Gibco, Waltham, MA), or Tumor Specialized Media with 2% FBS (wm3211). Human immortal melanocyte cell lines (Hermes 1, 3a and 4a) were generously provided by Professor Dorothy C. Bennett (University of London, UK). The culture media and conditions followed the directions as provided by The Wellcome Trust Functional Genomics Cell Bank²⁷ . Human IFN-γ was purchased from GoldBio (1160-06-100; St. Louis, MO) and IFN-α was obtained from PBL Assay Science (11100-1; Piscataway, NJ).