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Acell smart pool of sirna targeting mouse

Manufactured by Horizon Discovery
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The Acell SMART pool of siRNA targeting mouse is a laboratory equipment product designed for gene knockdown studies in mouse models. It consists of a pool of small interfering RNA (siRNA) molecules that collectively target a specific gene of interest in the mouse genome. The core function of this product is to provide researchers with a tool to efficiently silence the expression of a target gene and study its biological effects, without making claims about its intended use.

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Lab products found in correlation

Accell sirna delivery medium, by Horizon Discovery (2 mentions) Slc9a1 sirna, by Horizon Discovery (2 mentions) Alpha enac scnn1a, by Horizon Discovery (2 mentions)

Close spelling variants of this product

SiRNAs (161 citations) SiRNA pools (31 citations) SiRNA targeting (10 citations) Acell smart pool (2 citations)

2 protocols using acell smart pool of sirna targeting mouse

1

Splenic DCs siRNA Knockdown Assay

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Mouse splenic DCs were isolated using MACS. Four million cells were re-suspended in 1 mL of Accell siRNA delivery medium (GE Healthcare Dharmacon) supplemented with 2.5% FBS, 1 mM sodium pyruvate and 25 mM HEPES. Cells were incubated for 72 hr with 1 µM Acell SMART pool of siRNA targeting mouse NHE1 (Slc9a1 siRNA [E-048336-00-0005], or Alpha ENaC (Scnn1a [E-040678-00-0005] GE Healthcare Dharmacon). ON-TARGETplus Non-targeting Control siRNA (D-001810-01-05) was used as a negative control. Sodium chloride (40 mM) was added for additional 24 hr. The knockdown levels of Alpha ENaC and NHE1 were analyzed by western blot.
Barbaro N.R., Foss J.D., Kryshtal D.O., Tsyba N., Kumaresan S., Xiao L., Mernaugh R.L., Itani H.A., Loperena R., Chen W., Dikalov S., Titze J.M., Knollmann B.C., Harrison D.G, & Kirabo A. (2017). Dendritic Cell Amiloride-Sensitive Channels Mediate Sodium-Induced Inflammation and Hypertension. Cell reports, 21(4), 1009-1020.
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2

Splenic DCs siRNA Knockdown Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse splenic DCs were isolated using MACS. Four million cells were re-suspended in 1 mL of Accell siRNA delivery medium (GE Healthcare Dharmacon) supplemented with 2.5% FBS, 1 mM sodium pyruvate and 25 mM HEPES. Cells were incubated for 72 hr with 1 µM Acell SMART pool of siRNA targeting mouse NHE1 (Slc9a1 siRNA [E-048336-00-0005], or Alpha ENaC (Scnn1a [E-040678-00-0005] GE Healthcare Dharmacon). ON-TARGETplus Non-targeting Control siRNA (D-001810-01-05) was used as a negative control. Sodium chloride (40 mM) was added for additional 24 hr. The knockdown levels of Alpha ENaC and NHE1 were analyzed by western blot.
Barbaro N.R., Foss J.D., Kryshtal D.O., Tsyba N., Kumaresan S., Xiao L., Mernaugh R.L., Itani H.A., Loperena R., Chen W., Dikalov S., Titze J.M., Knollmann B.C., Harrison D.G, & Kirabo A. (2017). Dendritic Cell Amiloride-Sensitive Channels Mediate Sodium-Induced Inflammation and Hypertension. Cell reports, 21(4), 1009-1020.
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