Cobas fara semiautomatic analyzer

Muscle tissue, ~30 mg wet weight, was freeze dried, after which collagen, blood, and non–muscle fiber materials were removed from the muscle fibers under a microscope. The isolated muscle fiber mass (~5–7 mg) was weighed, and 500 μl of 1 M HCl was added. After heating for 3 h at 100°C to hydrolyze the glycogen to glycosyl units and cooling down to room temperature, 500 μl of the solution was neutralized by adding 280 μl of Tris–KOH (Tris 119 mM, KOH 2.14 M) and centrifuged at 1000 g and 4°C for 10 min. Thereafter, 150 μl of this solution was analyzed for glucose concentration (Glucose HK CP A11A01667, ABX Pentra) with a COBAS FARA semiautomatic analyzer (Roche).

Muscle tissue, ~30 mg wet weight, was freeze dried, after which collagen, blood, and nonmuscle fiber materials were removed from the muscle fibers under a microscope. The isolated muscle fiber mass (~5–7 mg) was weighed, and 500 μL of 1 MHCI was added. After heating for 3 h at 100°C to hydrolyze the glycogen to glycosyl units and cooling down to room temperature, 500 μL of the solution was neutralized by adding 280 μL of Tris–KOH (Tris 119 mmol/L, KOH 2.14 mol/L) and centrifuged at 1000 g and 4°C for 10 min. Thereafter, 150 μL of this solution was analyzed for glucose concentration (Glucose HK CP A11A01667, ABX Pentra) with a COBAS FARA semiautomatic analyzer (Roche).