The procedure of co-immunoprecipitation has been previously described (Sun et al., 2009 (link)). Briefly, equal amounts of protein extracts (250 μg) were incubated with GluA1 antibody (9 μg; Millipore, Billerica, MA) overnight, followed by the addition of TrueBlot anti-rabbit Ig immunoprecipitation beads (60 μL; eBio-science, San Diego, CA) for 4 hours at 4°C. Beads were washed 3 times, centrifuged, and then boiled in Lammeli buffer for 5 minutes for protein detachment. Proteins were loaded on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gels and analyzed for GluA1 and OTR by immunoblotting as described above. The primary antibody against OTR (1:500) was used to detect the association between GluA1 and OTR.
Glua1 antibody
Manufactured by Merck Group
The GluA1 antibody is a laboratory reagent used in research applications. It is a protein that binds specifically to the GluA1 subunit of the AMPA-type glutamate receptor, which is a key component of excitatory synaptic transmission in the central nervous system. The GluA1 antibody can be used to detect and quantify the presence of the GluA1 subunit in biological samples, such as cell lysates or tissue extracts, through techniques like Western blotting or immunohistochemistry.
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2 protocols using glua1 antibody
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Co-immunoprecipitation of GluA1 and OTR
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Co-immunoprecipitation of GluA1 and OTR
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The procedure of co-immunoprecipitation has been previously described (Sun et al., 2009 (link)). Briefly, equal amounts of protein extracts (250 µg) were incubated with GluA1 antibody (9 µg; Millipore, Billerica, MA) overnight, followed by the addition of TrueBlot anti-rabbit Ig immunoprecipitation beads (60 µL; eBioscience, San Diego, CA) for 4 hours at 4°C. Beads were washed 3 times, centrifuged, and then boiled in Lammeli buffer for 5 minutes for protein detachment. Proteins were loaded on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis gels and analyzed for GluA1 and OTR by immunoblotting as described above. The primary antibody against OTR (1:500) was used to detect the association between GluA1 and OTR.
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