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Rabbit anti brn3

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Rabbit anti-Brn3 is a primary antibody that specifically binds to the Brn3 transcription factor. Brn3 is a family of POU domain proteins that play important roles in neuronal development and function. This antibody can be used in various research applications to detect and study the expression of Brn3 in biological samples.

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Lab products found in correlation

Bx61 microscope, by Olympus (2 mentions) Goat anti mouse alexa 488, by Thermo Fisher Scientific (2 mentions) Rabbit anti sox2, by Abcam (2 mentions) Chicken anti rabbit alexa 594, by Thermo Fisher Scientific (2 mentions) Goat anti chicken alexa 594, by Thermo Fisher Scientific (2 mentions) Prolong gold, by Thermo Fisher Scientific (2 mentions) Hoechst 33342, by Merck Group (2 mentions) Sp5 2 microscope, by Leica (2 mentions) Goat anti mouse alexa 594, by Thermo Fisher Scientific (2 mentions) Goat anti chicken alexa 488, by Thermo Fisher Scientific (2 mentions) Chicken anti β gal, by Abcam (2 mentions) Rabbit anti β tubulin 3, by Fortrea (2 mentions) Goat anti rabbit alexa 488, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Rabbit anti-TM (2 citations)

2 protocols using rabbit anti brn3

1

Immunofluorescence Staining of Cryosectioned Eyes

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Eyes were harvested and fixed in 4% PFA for 2 hours prior to cryoprotection in 25% sucrose-PBS overnight. Eyes were embedded in OCT, and cryosectioned at 12 μm and mounted on SuperFrost Plus slides prior to immunofluorescence. Standard immunofluorescence procedures were followed.9 (link) Primary antibodies include: chicken anti-β-gal (Abcam Cambridge, MA; ab9361), rabbit anti-β-tubulin III (Covance, Princeton, NJ; PRB-435P), rabbit anti-Brn3 (Santa Cruz Biotechnology, Santa Cruz, CA; sc28595), and rabbit anti-Sox2 (Abcam; ab97959). Secondary antibodies used were (all from Invitrogen): chicken anti-rabbit Alexa-594, goat anti-chicken Alexa-488, goat anti-chicken Alexa-594, goat anti-mouse Alexa-488, goat anti-mouse Alexa-594, goat anti-rabbit Alexa-488, and goat anti-rabbit Alexa-594. Nuclei were stained using 1 μg/ml of Hoechst 33342 (Sigma, St Louis, MO; 14533). Slides were mounted with Prolong Gold (Invitrogen) antifade reagent and imaged using an Olympus BX61 microscope and the InVivo software (Olympus), or alternatively using confocal laser scanning microscopy with a Leica SP5 II microscope (Leica Microsystems) and LAF software (Leica Microsystems).
de Leeuw C.N., Dyka F.M., Boye S.L., Laprise S., Zhou M., Chou A.Y., Borretta L., McInerny S.C., Banks K.G., Portales-Casamar E., Swanson M.I., D’Souza C.A., Boye S.E., Jones S.J., Holt R.A., Goldowitz D., Hauswirth W.W., Wasserman W.W, & Simpson E.M. (2014). Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Molecular Therapy. Methods & Clinical Development, 1, 5-.
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2

Immunohistochemistry of Retinal Tissues

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Eyes were harvested and fixed in 4% PFA for 2 hours prior to cryoprotection in 25% sucrose-PBS overnight. Eyes were embedded in OCT, and cryosectioned at 12 μm and mounted on SuperFrost Plus slides prior to immunofluorescence. Standard immunofluorescence procedures were followed.9 (link) Primary antibodies include: chicken anti-β-gal (Abcam Cambridge, MA, ab9361), rabbit anti-β-tubulin III (Covance, Princeton, NJ, PRB-435P), rabbit anti-Brn3 (Santa Cruz Biotechnology, Santa Cruz, CA, sc28595), and rabbit anti-Sox2 (Abcam, ab97959). Secondary antibodies used were (Invitrogen): chicken anti-rabbit Alexa-594, goat anti-chicken Alexa-488, goat anti-chicken Alexa-594, goat anti-mouse Alexa-488, goat anti-mouse Alexa-594, goat anti-rabbit Alexa-488, goat anti-rabbit Alexa-594. Nuclei were stained using 1 μg/mL of Hoechst 33342 (Sigma, St. Louis, MO, 14533). Slides were mounted with Prolong Gold (Invitrogen) anti-fade reagent and imaged using an Olympus BX61 microscope and the InVivo software, or alternatively using confocal laser scanning microscopy with a Leica SP5 II microscope and LAF software.
de Leeuw C.N., Dyka F.M., Boye S.L., Laprise S., Zhou M., Chou A.Y., Borretta L., McInerny S.C., Banks K.G., Portales-Casamar E., Swanson M.I., D’Souza C.A., Boye S.E., Jones S.J., Holt R.A., Goldowitz D., Hauswirth W.W., Wasserman W.W, & Simpson E.M. (2014). Targeted CNS delivery using human MiniPromoters and demonstrated compatibility with adeno-associated viral vectors. Molecular Therapy. Methods & Clinical Development, 1, 5-.
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