Oxoid sr 147 supplement

Two biopsy samples were achieved from each patient from the antrum and the body of the stomach. The H. pylori isolates were cultured at 37 °C on Brucella agar plates (Becton Dickinson, Franklin Lakes, NJ, USA) containing 5% defibrinated sheep blood (Hanil Komed, Seongnam, Korea) under microaerobic conditions (5% O₂, 10% CO₂, 85% N₂) for 4 days. Organisms were identified as H. pylori by colony morphology, rapid urease test, H. pylori-selective media (Oxoid™ SR 147 supplement (Thermo Fisher Scientific, Waltham, MA) and 5% defibrinated sheep blood), and PCR to detect ureA. All stock cultures were stored at −70 °C in Brucella broth supplemented with 15% glycerol (Sigma Chemical Co., St Louis, MO, USA) and 10% fetal bovine serum (Gibco, Grand Island, NY, USA). These preparations were thawed and subcultured for further experiments.

H. pylori isolates were cultured at 37 °C on Brucella agar plates (Becton Dickinson, Franklin Lakes, NJ, USA) containing 5% defibrinated sheep blood (Hanil Komed, Seongnam, Republic of Korea) under microaerobic conditions (5% O₂, 10% CO₂, 85% N₂) for 3–5 days. The H. pylori isolates subcultured less than 3 times were used in all experiments, and subculture was carried out on blood Brucella agar plates under the same conditions. Organisms were identified as H. pylori by colony morphology, rapid urease test, H. pylori-selective media [Oxoid™ SR 147 supplement (Thermo Fisher Scientific, Waltham, MA) and 5% defibrinated sheep blood], and polymerase chain reaction (PCR) to detect ureA.
Genomic DNA extraction was performed by harvesting H. pylori subcultured for 3–5 days and using a HiYield™ genomic DNA mini kit (Real Biotech Corporation, Taipei, Taiwan). The isolated genomic DNA was stored at − 20 °C until required for PCR amplification.

During the endoscopic examination, two biopsy samples were taken from the antrum and the body of the stomach, from each patient. The H. pylori isolates were cultured at 37 °C for four days under microaerobic conditions (5% O₂, 10% CO₂, and 85% N₂) on Brucella agar plates (Becton Dickinson, Franklin Lakes, NJ, USA) supplemented with 5% defibrinated sheep blood (Hanil Komed, Seongnam, Republic of Korea) [33 (link)]. Identification of H. pylori was achieved by confirming typical colony morphology, positive rapid urease test, growth on H. pylori-selective media [Oxoid™ SR 147 supplement (Thermo Fisher Scientific, Waltham, MA, USA) and 5% defibrinated sheep blood], and detection of urea on PCR. Stock cultures were kept at −70 °C in Brucella broth containing 10% fetal bovine serum and 15% glycerol. For further experiments, these preparations were thawed and subcultured later.